Subcellular localization of CFTR to endosomes in a ductal epithelium.
Subcellular localization of CFTR to endosomes in a ductal epithelium.
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CFTR 亚细胞定位于导管上皮内体。
DOI：
10.1152/ajpcell.1994.267.2.c340
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Marino,CR
中科院分区：
文献类型：
--
作者：
Webster,P;Vanacore,L;Nairn,AC;Marino,CR
囊性纤维化跨膜电导调节器 (CFTR) 的质膜氯转运可能受到影响 CFTR 与质膜循环的细胞过程的调节。检验这一假设需要细胞化学证据来证明 CFTR 亚细胞区室的存在。在本研究中，使用免疫荧光和免疫电子显微镜对正常上皮细胞群中 CFTR 的亚细胞分布进行了表征。针对 CFTR 分子的不同表位产生的两种抗 CFTR 抗体，特异性标记大鼠颌下腺组织切片中横纹管上皮细胞的顶极。通过使用电子显微镜，CFTR 免疫反应性与顶端质膜和许多顶端下囊泡的膜相关。在该制剂中，一些 CFTR 标记的囊泡还标记有抗转铁蛋白受体和 rab4 的抗体，这是早期内体和受体介导的内吞作用的两个标记。这些观察结果为外周表达 CFTR 的内体的存在提供了直接的细胞化学证据，并支持膜回收可能有助于 CFTR 功能的假设。
Plasma membrane chloride transport by the cystic fibrosis transmembrane conductance regulator (CFTR) may be regulated by cellular processes that affect the cycling of CFTR with the plasma membrane. Testing this hypothesis requires cytochemical evidence for the presence of a subcellular compartment of CFTR. In this study, the subcellular distribution of CFTR in a normal epithelial cell population was characterized using immunofluorescence and immunoelectron microscopy. Two anti-CFTR antibodies, raised against different epitopes of the CFTR molecule, specifically labeled the apical pole of striated duct epithelial cells in tissue sections of rat submandibular gland. By use of electron microscopy, the CFTR immunoreactivity was associated with the apical plasma membrane and the membranes of many subapical vesicles. In this preparation, some of the CFTR-labeled vesicles were also labeled with antibodies against transferrin receptor and rab4, two markers of early endosomes and receptor-mediated endocytosis. These observations provide direct cytochemical evidence for the existence of peripherally located CFTR-expressing endosomes and support the hypothesis that membrane recycling may contribute to CFTR function.