Differentiation of the pericyte in wound healing: The precursor, the process, and the role of the vascular endothelial cell
Differentiation of the pericyte in wound healing: The precursor, the process, and the role of the vascular endothelial cell
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DOI：
10.1111/j.1524-475x.2008.00374.x
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发表时间：
2008-05-01
影响因子：
2.9
通讯作者：
Li Jinqing
中科院分区：
文献类型：
--
作者：
Li Xueyong;Chen Shaozong;Li Jinqing
周细胞在新形成血管的稳态和成熟中发挥着至关重要的作用，但其前体和分化过程仍不清楚。在这项研究中，我们发现，在体内，取自烧伤患者的人肉芽组织中的周细胞表达 CD13 和 I 型胶原蛋白，它们是外周血纤维细胞 (PBFC) 的细胞标志物。用 PKH-26 离体标记并静脉注射回小鼠体内的小鼠 PBFC 在肉芽组织中形成管腔并表达周细胞标记物 NG2。此外，在细胞培养中，与人脐血管内皮细胞或人真皮微血管内皮细胞（HDMEC）共培养的人PBFC表达平滑肌细胞周细胞标记结蛋白和肌球蛋白重链，并且部分迁移至人脐血管内皮细胞或HDMEC细胞膜上形成PBFC/血管 内皮细胞簇，或仅形成圆形 PBFC 簇，其细胞核沿圆圈排列。 PBFC/血管内皮细胞簇和圆形PBFC簇的形成受到单核细胞趋化蛋白1抗体的抑制。用肉芽组织匀浆培养的PBFC也表达平滑肌细胞的结蛋白和肌球蛋白重链。我们的研究结果支持PBFC是周细胞的前体，PBFC的分化是由血管内皮细胞以旁分泌方式诱导的。
Pericytes play a crucial role in the homeostasis and maturation of newly formed vessels, but their precursor and the process of their differentiation remain unclear. In this study, we show that in vivo, pericytes in human granulation tissue taken from burn patients expressed CD13 and collagen I, which are cell markers of peripheral blood fibrocytes (PBFCs). Mouse PBFCs, ex vivo labeled by PKH-26 and administered intravenously back to mice, formed lumens in the granulation tissue and expressed pericyte marker NG2. Furthermore, in cell culture, human PBFCs in co-culture with human umbilical vascular endothelial cells or human dermal microvascular endothelial cells (HDMECs) expressed pericyte markers desmin and myosin heavy chain of smooth muscle cell, and some migrated to the membrane of human umbilical vascular endothelial cells or HDMECs to form PBFC/vascular endothelial cell clusters, or formed round PBFC only clusters with their nuclei aligned along a circle. The formation of PBFC/vascular endothelial cell clusters and round PBFC clusters were inhibited by an antibody against monocyte chemotactic protein-1. PBFCs cultured with homogenate of granulation tissue also expressed desmin and myosin heavy chain of smooth muscle cell. Our findings support that the PBFC is a precursor of pericytes, the differentiation of PBFCs is induced by vascular endothelial cells in a paracrine fashion.