Chromosomes are predominantly located randomly with respect to each other in interphase human cells
Chromosomes are predominantly located randomly with respect to each other in interphase human cells
复制标题
DOI：
10.1083/jcb.200206009
复制
发表时间：
2002-10-28
影响因子：
7.8
通讯作者：
Brenner, DJ
中科院分区：
文献类型：
--
作者：
Cornforth, MN;Greulich-Bode, KM;Brenner, DJ
为了定量测试是否有系统的染色体-染色体协会在人类间期细胞核，所有可能的异源染色体对之间的互换进行了测量与24色全染色体彩绘（多重FISH），后损伤间期淋巴细胞稀疏电离辐射在体外。特定染色体对的过量互换将指示构成该对的染色体之间的空间接近性。实验设计是这样的，从随机性（一组染色体内的额外成对交换）的相当小的偏差将是可检测的。唯一具有统计学意义的染色体簇是先前观察到的优先位于细胞核中心附近的一组5条染色体。然而，从数量上讲，整个基因组内与随机性的总体偏差很小。因此，虽然一些染色体染色体协会是明确存在的，在整个染色体水平上，占主导地位的整体模式似乎是空间随机的。
To test quantitatively whether there are systematic chromosome-chromosome associations within human interphase nuclei, interchanges between all possible heterologous pairs of chromosomes were measured with 24-color whole-chromosome painting (multiplex FISH), after damage to interphase lymphocytes by sparsely ionizing radiation in vitro. An excess of interchanges for a specific chromosome pair would indicate spatial proximity between the chromosomes comprising that pair. The experimental design was such that quite small deviations from randomness (extra pairwise interchanges within a group of chromosomes) would be detectable. The only statistically significant chromosome cluster was a group of five chromosomes previously observed to be preferentially located near the center of the nucleus. However, quantitatively, the overall deviation from randomness within the whole genome was small. Thus, whereas some chromosome-chromosome associations are clearly present, at the whole-chromosomal level, the predominant overall pattern appears to be spatially random.