DNA-REPLICATION TRIGGERED BY DOUBLE-STRANDED BREAKS IN ESCHERICHIA-COLI - DEPENDENCE ON HOMOLOGOUS RECOMBINATION FUNCTIONS
DNA-REPLICATION TRIGGERED BY DOUBLE-STRANDED BREAKS IN ESCHERICHIA-COLI - DEPENDENCE ON HOMOLOGOUS RECOMBINATION FUNCTIONS
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DOI：
10.1016/0092-8674(94)90279-8
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发表时间：
1994-09-23
期刊：
影响因子：
64.5
通讯作者：
KOGOMA, T
中科院分区：
文献类型：
--
作者：
ASAI, T;BATES, DB;KOGOMA, T
携带λ cos位点质粒的同源重组依赖性DNA复制（RDR）在E.当细胞表达将双链断裂引入COS位点的λ末端酶时，如果质粒也含有重组热点chi，则RDR发生在正常野生型细胞中。当细胞被诱导为SOS时，chi被抑制。反应或含有recD突变。recBC sbcA突变体细胞也能够诱导RDR。recN突变大大降低了正常细胞中的RDR，但在SOS诱导的细胞中则不然。RDR通过DNA合成的θ模式或滚环模式进行，分别产生共价闭合的环状质粒单体或线性质粒多聚体。先前描述的可诱导的稳定DNA复制被认为是一种特殊类型的RDR，其仅从染色体上的特定位点（oriM）开始。
Homologous recombination-dependent DMA replication (RDR) of a lambda cos site-carrying plasmid is demonstrated in E. coli cells when the cells express lambda terminase that introduces a double-stranded break into the cos site. RDR occurs in normal wild-type cells if the plasmid also contains the recombination hotspot chi. chi is dispensable when cells are induced for the SOS. response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction. A recN mutation greatly reduces RDR in normal cells, but not in SOS-induced cells. RDR proceeds by the theta mode or rolling circle mode of DNA synthesis, yielding covalently closed circular plasmid monomers or linear plasmid multimers, respectively. Previously described inducible stable DNA replication is considered to be a special type of RDR that starts exclusively from specific sites (oriMs) on the chromosome.