Chemosensory proteins participate in insecticide susceptibility in Rhopalosiphum padi, a serious pest on wheat crops
Chemosensory proteins participate in insecticide susceptibility in Rhopalosiphum padi, a serious pest on wheat crops
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化学感应蛋白参与小麦作物严重害虫稻盘丝虫的杀虫剂敏感性
DOI：
10.1111/imb.12683
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发表时间：
2020-12-14
影响因子：
2.6
通讯作者：
Chen, M. H.
中科院分区：
文献类型：
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作者：
Peng, X.;Qu, M. J.;Chen, M. H.
稻纵卷叶蝉是一种世界性的农业害虫。化学感觉蛋白(CSP)被认为是一种能与外界环境中的化学物质结合的转运蛋白。以往的研究表明，一些昆虫在接触杀虫剂后CSPs的表达显著增加，CSPs参与了对杀虫剂的抗性或敏感性。然而，CSPs在P.PADI对杀虫剂的敏感性和反应中的作用仍不清楚。在本研究中，我们通过全基因组研究鉴定了8个RPADI的CSP(RpCSP)。7个RpCSP基因有2个外显子，而RpCSP7基因有3个外显子。定量聚合酶链式反应分析表明，吡虫啉和高效氯氰菊酯在不同处理时间对8个RpCSP基因的mRNA水平有显著影响。分子对接预测在RpCSP4、RpCSP5、RpCSP6、RpCSP7和RpCSP10与吡虫啉和高效氯氰菊酯的结合过程中存在起关键作用的氢键。利用RNA干扰技术将RpCSP4、RpCSP5、RpCSP6和RpCSP10基因敲除后，在两种亚致死浓度的吡虫啉作用下均能显著提高棉铃虫的死亡率。在两种亚致死浓度的高效氯氰菊酯条件下，注射含有dsCSP4和dsCSP6的R.PADI后，死亡率显著升高。结果表明，部分RpCSP基因参与了根结线虫对杀虫剂的敏感性。
Rhopalosiphum padi is a worldwide agricultural pest. Chemosensory proteins (CSPs) are considered to be a type of transporters which can bind chemicals from external environments. Previous research showed that the expression of some insect CSPs were significantly increased after exposure to insecticides, and CSPs were involved in insecticide resistance or susceptibility. However, the role of CSPs in the susceptibility and response of R. padi to insecticides is still unknown. In this study, we identified eight CSP (RpCSP) from R. padi by genome‐wide investigation. Seven RpCSP genes had two exons, while RpCSP7 had three exons. qPCR analyses showed that the mRNA levels of the eight RpCSP genes were significantly affected by imidacloprid and beta‐cypermethrin in different post‐treatment periods. Molecular docking predicted that there were hydrogen bonding sites which played key roles in binding of RpCSP4, RpCSP5, RpCSP6, RpCSP7 and RpCSP10 with imidacloprid and beta‐cypermethrin. Knockdown of RpCSP4, RpCSP5, RpCSP6 and RpCSP10 by RNA interference significantly increased the aphid mortality under two sublethal concentrations of imidacloprid. Mortalities under two sublethal concentrations of beta‐cypermethrin conditions were significantly higher after injection of R. padi with dsCSP4 and dsCSP6. The results indicate that some RpCSP genes are involved in the insecticide susceptibility of R. padi.