Multitarget super-resolution microscopy with high-density labeling by exchangeable probes
Multitarget super-resolution microscopy with high-density labeling by exchangeable probes
复制标题
DOI：
10.1038/nmeth.3466
复制
发表时间：
2015-08-01
期刊：
影响因子：
48
通讯作者：
Watanabe, Naoki
中科院分区：
文献类型：
--
作者：
Kiuchi, Tai;Higuchi, Makio;Watanabe, Naoki
我们开发了一种多目标超分辨率显微技术，称为通过整合可交换单分子定位(IRIS)进行图像重建。虹膜使用基于蛋白质片段的探针，在数十毫秒量级的持续时间内与其目标直接关联和分离。通过将单分子定位和顺序标记相结合，IRIS能够实现沿多个细胞结构的前所未有的标记密度。虹膜可以用来辨别单个细胞内细胞骨架组件和局部粘连之间的特定区域接近程度。
We have developed a multitarget super-resolution microscopy technique called image reconstruction by integrating exchangeable single-molecule localization (IRIS). IRIS uses protein fragment-based probes that directly associate with and dissociate from their targets over durations on the order of tens of milliseconds. By integrating single-molecule localization and sequential labeling, IRIS enables unprecedented labeling density along multiple cellular structures. IRIS can be used to discern the area-specific proximity between cytoskeletal components and focal adhesions within a single cell.