An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3.
An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3.
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DOI：
10.1111/j.1574-6968.2009.01481.x
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发表时间：
2009-04
影响因子：
2.1
通讯作者：
Peng Zhang;P. Zhou;Longjiang Yu
中科院分区：
文献类型：
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作者：
Peng Zhang;P. Zhou;Longjiang Yu
从曼地亚红豆杉内树皮中分离到一株产紫杉醇的内生真菌(菌株MD3)，经形态特征、生理生化特性和18S rRNA基因序列分析鉴定为白曲霉。紫杉醇经UV、HPL C、MS和核磁共振波谱分析表明，其产物紫杉醇与正品紫杉醇完全相同。10-脱乙酰巴卡菌素III-10-O-乙酰基转移酶是紫杉醇生物合成途径中催化形成最后一个二萜中间体的基因，该基因是首次从念珠菌MD3中克隆得到的，与红豆杉中发现的基因具有很高的同源性。
An endophytic taxol-producing fungus (strain MD3) isolated from the inner bark of Taxus x media was identified as Aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18S rRNA gene sequence analysis. Taxol produced by A. candidus MD3 was shown to be identical to authentic taxol analyzed by UV, HPLC, MS and nuclear magnetic resonance spectroscopy. The gene encoding the 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the taxol biosynthetic pathway, has been cloned from A. candidus MD3 for the first time and possesses high homology to the same gene found in Taxus spp.