Clinical and molecular change induced by repeated low-dose visible light exposure in both light-skinned and dark-skinned individuals.
Clinical and molecular change induced by repeated low-dose visible light exposure in both light-skinned and dark-skinned individuals.
复制标题
浅色皮肤和深色皮肤个体重复低剂量可见光照射引起的临床和分子变化。
DOI：
10.1111/phpp.12819
复制
发表时间：
2023
期刊：
影响因子：
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通讯作者：
Chien,AnnaL
中科院分区：
文献类型：
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作者：
Kim,Sooyoung;Rainer,BarbaraM;Qi,Ji;Brown,Isabelle;Ogurtsova,Aleksandra;Leung,Sherry;Garza,LuisA;Kang,Sewon;Chien,AnnaL
BackgroundVisible light (VL) is known to induce pigmentation in dark‐skinned individuals and immediate erythema in light‐skinned individuals. However, the effects of accumulated low‐dose VL exposure across skin types are not well established.MethodsThirty‐one healthy subjects with light (Fitzpatrick skin types [FST] I‐II,n= 13) and dark (FST V‐VI,n= 18) skin types were enrolled. Subjects' buttocks were exposed daily to VL, wavelength 400–700 nm, with a dose of 120 J/cm2at 50 mW/cm2, for four consecutive days. Microarray using Affymetrix GeneChip (49,395 genes) was performed followed by qRT‐PCR on skin samples.ResultsRepeated low‐dose VL irradiation induced immediate pigment darkening and delayed tanning in dark‐skinned individuals while no discernable pigmentation and erythema were observed in light‐skinned individuals. Top ten upregulated genes by repeated VL exposure in microarray included melanogenic genes such as tyrosinase (TYR), tyrosinase‐related protein‐1 (TYRP1), dopachrome tautomerase (DCT), premelanosome protein (PMEL), melan‐A (MLANA), and solute carrier family 24, member 5 (SLC24A5) and genes involved in inflammation/matrix remodeling/cell signaling including chemokine (C‐C motif) ligand 18 (CCL18), BCL2‐related protein A1 (BCL2A1), and cartilage oligomeric matrix protein (COMP). In qRT‐PCR CCL18 was upregulated in light skin with a greater extent (mean fold change ± SD; 4.03 ± 3.28,p= .04) than in dark‐skinned individuals (1.91 ± 1.32,p= .07) while TYR was not significantly upregulated in both skin types.ConclusionThis study highlights the genes upregulated by cumulative VL exposure involved in pigmentation, immune response, oxidation/reduction, and matrix remodeling across skin types providing relevant information on daily solar exposure.
