Bio-nanocapsule conjugated with liposomes for in vivo pinpoint delivery of various materials
Bio-nanocapsule conjugated with liposomes for in vivo pinpoint delivery of various materials
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DOI：
10.1016/j.jconrel.2007.12.002
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发表时间：
2008-03-20
影响因子：
10.8
通讯作者：
Kuroda, Shun'ichi
中科院分区：
文献类型：
--
作者：
Jung, Joohee;Matsuzaki, Takashi;Kuroda, Shun'ichi
由乙型肝炎病毒（HBV）表面抗原（HBsAg）组成的生物纳米胶囊（bnc）是一种约50纳米的中空颗粒，具有人类肝细胞识别分子（前s1肽）。在过去的二十年里，它们一直被用作乙肝疫苗。原始BNC可以通过电穿孔结合各种有效载荷（如药物、基因），并利用HBV感染机制特异性地将其递送到人肝细胞。在这里，我们开发了一种新的BNC与脂质体结合，并成功地将大材料（100 nm荧光标记的聚苯乙烯珠和bbb30 kbp质粒）纳入BNC-脂质体复合物中。这种复合物在体内和体外都能将这些大的物质输送到人肝细胞中。BNC-脂质体复合物转染效率显著高于原BNC。这些结果表明，BNC赋予了传统脂质体的组织和细胞特异性，并为体内药物传递系统、基因传递系统和生物成像系统提供了新的可能性。(c) 2007 Elsevier B.V.版权所有
Bio-nanocapsules (BNCs) consisting of hepatitis B virus (HBV) surface antigen (HBsAg) are approximately 50-nm hollow particles displaying a human hepatocyte-recognizing molecule (pre-S1 peptide). They have been used as an HB vaccine for the last two decades. Original BNC can incorporate various payloads (e.g., drugs, genes) by electroporation and deliver them to human hepatocytes specifically by utilizing the HBV infection mechanism. Here, we developed a new BNC conjugated with liposomes and succeeded in incorporating large materials (100-nm fluorescence-labeled polystyrene beads and > 30 kbp plasmids) into the BNC-liposome complex. The complex delivered these large materials to human hepatocytes specifically ex vivo and in vivo. The transfection efficiency of the BNC-liposome complex was significantly higher than that of the original BNC. These results indicated that BNC confers the tissue- and cell-specificity on the conventional liposomes and raises new possibilities for drug delivery systems, gene delivery systems, and bio-imaging systems in vivo. (c) 2007 Elsevier B.V. All rights reserved.