Experimental research of host macrophage canceration induced by glioma stem progenitor cells.
Experimental research of host macrophage canceration induced by glioma stem progenitor cells.
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胶质瘤干祖细胞诱导宿主巨噬细胞癌变的实验研究
DOI：
10.3892/mmr.2014.3032
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发表时间：
2015-04
影响因子：
3.4
通讯作者：
Huang Q
中科院分区：
文献类型：
--
作者：
Wang A;Dai X;Cui B;Fei X;Chen Y;Zhang J;Zhang Q;Zhao Y;Wang Z;Chen H;Lan Q;Dong J;Huang Q
肿瘤相关巨噬细胞参与肿瘤进展是不争的事实。然而，巨噬细胞对侵袭期肿瘤的生长促进作用是否会影响其自身的癌变仍不清楚。本研究将转染红色荧光蛋白基因（SU3-RFP）的人胶质瘤干/祖细胞接种于转基因裸鼠腹腔内，其中所有有核细胞均可表达绿色荧光蛋白（GFP），形成双色RFP/GFP荧光示踪剂的肿瘤模型。培养荷瘤小鼠的腹水和肿瘤结节，然后分离GFP+细胞进行克隆培养，并进一步进行相关的表型表征和致瘤性测试。观察到从腹水和实体瘤中分离的GFP+细胞表现出无限的增殖潜力；这些单克隆细胞是小鼠来源的，具有癌细胞表型并表达巨噬细胞标记蛋白CD68。因此，在双色荧光示踪剂的腹部肿瘤模型中，肿瘤细胞招募的巨噬细胞不仅促进肿瘤细胞生长，而且还表现出自身的癌变。这一发现对于进一步研究肿瘤组织重塑和肿瘤微环境具有重要意义。
The involvement of tumor-associated macrophages in tumor progression is an indisputable fact. However, whether the growth-promotion effects of macrophages towards tumors in the aggressive stage affect their own canceration remains unknown. In the present study, human glioma stem/progenitor cells transfected with red fluorescent protein gene (SU3-RFP) were seeded inside the abdominal cavity of transgenic nude mice, of which all nucleated cells could express green fluorescent protein (GFP), forming a tumor model with a double-color RFP/GFP fluorescent tracer. Ascites and tumor nodules from tumor-bearing mice were cultured, then the GFP+ cells were separated for clonal culture and further related phenotypic characterization and tumorigenicity tests. It was observed that the GFP+ cells isolated from ascites and solid tumors exhibited unlimited proliferative potential; the monoclonal cells were mouse-original, had a cancer cell phenotype and expressed the macrophage marker protein CD68. Thus, in the abdominal tumor model with double-color fluorescent tracer, macrophages recruited by tumor cells not only promoted tumor cell growth, but also exhibited their own canceration. This discovery is significant for the further study of tumor tissue remodeling and the tumor microenvironment.
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影响因子：
3.7
作者：
Goldenberg DM;Gold DV;Loo M;Liu D;Chang CH;Jaffe ES
通讯作者：
Jaffe ES
影响因子：
64.5
作者：
Hou, Yong;Song, Luting;Wang, Jun
通讯作者：
Wang, Jun
影响因子：
11.2
作者：
Garcia-Olmo, Dolores C.;Dominguez, Carolina;Garcia-Olmo, Damian
通讯作者：
Garcia-Olmo, Damian
建立绿色荧光蛋白示踪小鼠模型，重点关注宿主成分在坏死修复中的功能和皮下植入神经胶质瘤的生态位。
影响因子：
4.2
作者：
Lu, Zhao-Hui;Lv, Ke;Huang, Qiang
通讯作者：
Huang, Qiang
影响因子：
11.2
作者：
Lin, Elaine Y.;Li, Jiu-Feng;Pollard, Jeffrey W.
通讯作者：
Pollard, Jeffrey W.