3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification.
3D expression patterns of cell cycle genes in the developing chick wing and comparison with expression patterns of genes implicated in digit specification.
复制标题
发育中的鸡翅中细胞周期基因的 3D 表达模式以及与数字规范相关基因的表达模式的比较。
DOI：
10.1002/dvdy.22633
复制
发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Welten M
中科院分区：
文献类型：
--
作者：
Welten M
Sonic hedgehog （Shh）信号控制着小鸡翅膀趾型和生长的综合规范，但下游基因网络仍未被揭示。我们使用光学投影断层扫描分析了编码细胞周期调节因子的基因的三维表达模式。基因表达空间矩阵的分层聚类揭示了翼芽的背层，几乎所有基因都在其中表达，编码阳性细胞周期调节因子的基因具有相似的表达模式，而编码n - mycandcyclind2的基因表达模式不同但密切相关。我们将这些模式与涉及数字规范的基因和ptch1的基因进行了计算比较，总共有50个基因。19个基因具有相似的后表达toPtch1，包括hoxd13、Sall1、Hoxd11和bmp2，它们都可能是小鼠肢体中Gli的靶点，以及细胞周期基因N - myc、CyclinD2。我们认为，这些基因促成了一个整合数字规范和生长的网络，以响应Shh。科学通报，2011(4):387 - 387。©2011 Wiley‐Liss, Inc。
Sonic hedgehog (Shh) signalling controls integrated specification of digit pattern and growth in the chick wing but downstream gene networks remain to be unravelled. We analysed 3D expression patterns of genes encoding cell cycle regulators using Optical Projection Tomography. Hierarchical clustering of spatial matrices of gene expression revealed a dorsal layer of the wing bud, in which almost all genes were expressed, and that genes encoding positive cell cycle regulators had similar expression patterns while those ofN‐mycandCyclinD2were distinct but closely related. We compared these patterns computationally with those of genes implicated in digit specification andPtch1, 50 genes in total. Nineteen genes have similar posterior expression toPtch1, includingHoxd13, Sall1, Hoxd11, andBmp2, all likely Gli targets in mouse limb, and cell cycle genes,N‐myc,CyclinD2. We suggest that these genes contribute to a network integrating digit specification and growth in response to Shh. Developmental Dynamics 240:1278–1288, 2011. © 2011 Wiley‐Liss, Inc.