Immunohistochemical expressions of Ki-67, cyclin D1, β-catenin, cyclooxygenase-2, and epidermal growth factor receptor in human colorectal adenoma:: A validation study of tissue microarrays
Immunohistochemical expressions of Ki-67, cyclin D1, β-catenin, cyclooxygenase-2, and epidermal growth factor receptor in human colorectal adenoma:: A validation study of tissue microarrays
复制标题
DOI：
10.1158/1055-9965.epi-05-0946
复制
发表时间：
2006-09-01
影响因子：
3.8
通讯作者：
Zheng, Wei
中科院分区：
文献类型：
--
作者：
Su, Yinghao;Shrubsole, Martha J.;Zheng, Wei
背景：组织微阵列（TMA）有望成为一种高通量的生物标志物分析方法。然而，该方法的有效性和可靠性可能因不同组织样本中的不同生物标志物而有所不同。目的：在这项研究中，我们评估的有效性和可靠性，使用TMA评估生物标志物在结直肠adenomas.Methods：63例连续结直肠腺瘤患者被招募在这项研究中。使用来自每个腺瘤的四个穿孔构建两个TMA块（一个外围区、一个深部区和两个中间区）。免疫组化的五个标志物（Ki-67，细胞周期蛋白D1，β-连环蛋白，环氧合酶-2，表皮生长因子受体）进行了分析，并从TMAs和标准的全组织切片获得的数据之间的一致性进行了评价斯皮尔曼的相关性和kappa analysis.Results：大肠腺瘤呈带状，异质性表达模式的所有五个标志物。从TMAs和整个切片的数据之间的一致率为87%至93%，相应的kappa统计值为77%至90%的标记物的serniquantitative评价。此外，定量和半定量两种方法对TMA切片进行评分，两种方法对所有5种标记物的评分均显示出良好的相关性，组内相关系数在0.5 ~ 0.8之间。我们的研究表明，TMA可用于可靠地评估Ki-67、细胞周期蛋白131、β-连环蛋白、环氧合酶-2、和表皮生长因子受体的表达。
Background: Tissue microarray (TMA) holds promise as a high-throughput method for the analysis of biomarkers in tissue specimens. The validity and reliability of this method, however, may vary for different biomarkers in different tissue specimens. Objectives: In this study, we evaluated the validity and reliability of using TMA to assess biomarkers in colorectal adenomas.Methods: Sixty-three consecutive patients with colorectal adenomas were recruited in this study. Two TMA blocks were constructed using four punches from each adenoma (one periphery, one deep, and two middle zones). The immunostaining of five markers (Ki-67, cyclin D1, beta-catenin, cyclooxygenase-2, and epidermal growth factor receptor) was analyzed, and the concordance between data obtained from TMAs and standard whole-tissue sections was evaluated by Spearman's correlation and kappa analysis.Results: Colorectal adenoma exhibited zonal, heterogeneous expression patterns for all five markers. The concordance rates for the serniquantitative evaluation of markers between data from TMAs and whole sections ranged from 87% to 93% with corresponding kappa statistics of 77% to 90%. In addition, both quantitative and serniquantitative methods were used to score TMA sections, and good correlations between these two methods were shown for all five markers with intraclass correlation coefficients ranging from 0.5 to 0.8.Conclusion: Our study indicates that TMA can be used to reliably assess the expression levels of Ki-67, cyclin 131, beta-catenin, cyclooxygenase-2, and epidermal growth factor receptor in colorectal adenoma tissues.