Intranasal immunization with a live Streptococcus suis isogenic ofs mutant elicited suilysin-neutralization titers but failed to induce opsonizing antibodies and protection
Intranasal immunization with a live Streptococcus suis isogenic ofs mutant elicited suilysin-neutralization titers but failed to induce opsonizing antibodies and protection
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DOI：
10.1016/j.vetimm.2009.05.009
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发表时间：
2009-12-15
影响因子：
1.8
通讯作者：
Baums, Christoph Georg
中科院分区：
文献类型：
--
作者：
Kock, Christoph;Beineke, Andreas;Baums, Christoph Georg
在欧洲，猪链球菌血清型2和9是仔猪脑膜炎、关节炎和败血症的重要病原体。到目前为止，还没有描述过疫苗可以增强对这两种血清型的保护。这项研究的工作假设是，一个同基因血清2型突变体的毒力减弱，但不是在呼吸道定植可能会诱导保护性免疫对这两种血清型。用减毒沙门氏菌免疫仔猪。suis血清2型菌株10 Delta of s2/12，其缺乏血清不透明因子OFS的表达。在鼻内施用10个Delta of s2/12仔猪后三周，用代表最重要的S.欧洲的猪致病型，同源MRP+ EF+ SLY+血清型2株和异源MRP* SLY+血清型9株。活疫苗的应用引起了显着的血清IgG抗体对溶菌酶释放蛋白（MRP），细胞外因子（EF），最突出的是，suilysin的反应。在接种组中，抗猪溶血素的血清转化伴随着猪溶血素中和滴度的增加。尽管接种组的死亡率较低，但未观察到针对同源或异源攻击的显著保护。这与接种仔猪的低调理抗体滴度不足以介导猪中性粒细胞消除两种攻毒株的结果一致。总之，单次鼻内应用S.猪2型10 Δ ofs 2/12株可诱导不同类型猪链球菌的体液免疫应答。但未能诱导足够的调理抗体滴度和针对全身血清型2和9攻击的保护性免疫。(C)2009 Elsevier B. V.保留所有权利。
In Europe, Streptococcus suis serotypes 2 and 9 are very important causative agents of meningitis, arthritis and septicemia in piglets. So far, no vaccine has been described which elicits protection against both serotypes. The working hypothesis of this study was that an isogenic serotype 2 mutant attenuated in virulence but not in colonization of the respiratory tract might induce protective immunity against both serotypes. Piglets were immunized with the attenuated S. suis serotype 2 strain 10 Delta ofs2/12, which is deficient in expression of the serum opacity factor OFS. Three weeks after intranasal application of 10 Delta ofs2/12 piglets were challenged intravenously with two strains representing the most important S. suis pathotypes in Europe, the homologous MRP+ EF+ SLY+ serotype 2 strain and a heterologous MRP* SLY+ serotype 9 strain. Application of the live vaccine elicited significant serum IgG responses against muramidase-released protein (MRP), extracellular factor (EF) and, most prominently, suilysin. Seroconversion against suilysin was accompanied with an increase of suilysin-neutralization titers in the vaccinated group. Though mortality was lower in the vaccinated groups, significant protection was not observed, neither against the homologous nor against the heterologous challenge. This was in agreement with the finding that the vaccinated piglets had low opsonizing antibody titers insufficient to mediate elimination of the two challenge strains by porcine neutrophils. In conclusion, a single intranasal application of the S. suis serotype 2 strain 10 Delta ofs2/12 elicited humoral immune responses against different S. suis antigens but failed to induce sufficient opsonizing antibody titers and protective immunity against systemic serotypes 2 and 9 challenges. (C) 2009 Elsevier B.V. All rights reserved.