Polymeric optical microscreen for high-resolution surface plasmon resonance microarray imaging
Polymeric optical microscreen for high-resolution surface plasmon resonance microarray imaging
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DOI：
10.1021/la7014836
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发表时间：
2007-09-25
期刊：
影响因子：
3.9
通讯作者：
Hyun, Jinho
中科院分区：
文献类型：
--
作者：
Jung, Jaeyeon;Yuk, Jong Seol;Hyun, Jinho
在这项研究中，我们展示了一种简单的方法来制造表面等离子体共振（SPR）成像微阵列使用聚合物微图案。微米级聚合物光学屏幕（microPOS）的使用通过完全去除SPR信号或通过饱和远远超出SPR成像检测范围的SPR信号来钝化沉积有聚合物的区域。提出了两种方案，通过在沉积金属层之前（完全去除SPR）或在沉积金属层之后（SPR信号饱和）对厚绝缘层进行微图案化来创建表面microPOS。这两种方案被成功地应用于生物吸附的成像，具有类似于100 μ m/图案和10 μ m分离的高成像分辨率。以生物素-链霉亲和素系统为模型，验证了该系统的有效性。此外，前列腺特异性抗原（PSA）结合到抗PSA SPR微阵列上被证明是用于检测癌症标志物的有用方法。
In this study, we demonstrate a simple method to fabricate surface plasmon resonance (SPR) imaging microarrays using polymer micropatterns. The use of a micrometer-scale polymeric optical screen (microPOS) passivates the region deposited with polymer by completely removing SPR signals or by saturating the SPR signal far beyond the detection range of SPR imaging. Two schemes were suggested to create a surface microPOS by either micropatterning a thick insulating layer before deposition of a metal layer (complete removal of SPR) or after deposition of a metal layer (saturation of SPR signal). The two schemes were successfully applied for the imaging of biological adsorption with a high imaging resolution of similar to 100 mu m/pattern and 10 mu m separation. The validity of the system was verified with a biotin-streptavidin system as a model for the systematic binding of biomolecules. Further, binding of prostate-specific antigen (PSA) onto the anti-PSA SPR microarray was demonstrated as a useful method for detecting a cancer marker.