IMMUNOCYTOCHEMICAL DEMONSTRATION OF TOPOGRAPHIC ORDERING OF PURKINJE-CELL AXON TERMINALS IN THE FASTIGIAL NUCLEI OF THE RAT
IMMUNOCYTOCHEMICAL DEMONSTRATION OF TOPOGRAPHIC ORDERING OF PURKINJE-CELL AXON TERMINALS IN THE FASTIGIAL NUCLEI OF THE RAT
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DOI：
10.1002/cne.902440406
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发表时间：
1986-02-22
影响因子：
2.5
通讯作者：
LECLERC, N
中科院分区：
文献类型：
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作者：
HAWKES, R;LECLERC, N
我们使用一种单克隆抗体，mabQ113，选择性地染色小脑浦肯野细胞亚群，来研究大鼠正中蚓到尖状核的皮质核投影的地形。顶突投影区包含mabQ113+和mabQ113-浦肯野细胞，它们被分成副矢状带。免疫反应性遍及整个浦肯野细胞，包括轴突末端，因此可以研究mabQ113+末端的地形分布。在顶突核中，接收mabQ113+轴突末端的靶细胞集中在尾极。在吻侧极，细胞接受抗gad +末端，但不接受mabQ113+末端。抗gad染色无梯度。mabQ113、抗gad和抗突触抗体mabQ155的双重标记实验表明，在同一目标神经元上，mabQ113+和mabQ113-浦肯野细胞末端很少或没有混合。
We have used a monoclonal antibody, mabQ113, which selectively stains a subset of cerebellar Purkinje cells, to study the topography of the corticonuclear projection of the median vermis to the fastigial nuclei in the rat. The fastigial projection zone contains both mabQ113+ and mabQ113- Purkinje cells grouped into parasagittal bands. The immunoreactivity extends throughout the Purkinje cell including the axon terminals and thus it is possible to investigate the topographic distribution of mabQ113+ terminals. In the fastigial nuclei the target cells receiving mabQ113+ axon terminals are concentrated in the caudal pole. In the rostral pole, cells receive anti-GAD+ terminals but not mabQ113+ terminals. There is no gradient in anti-GAD staining. Double-labelling experiments with mabQ113, anti-GAD, and an antisynaptic antibody mabQ155 suggest that there is little or no mixing of mabQ113+ and mabQ113- Purkinje cell terminals on the same target neuron.