Structural characterization of proteins and complexes using small-angle X-ray solution scattering
Structural characterization of proteins and complexes using small-angle X-ray solution scattering
复制标题
DOI：
10.1016/j.jsb.2010.06.012
复制
发表时间：
2010-10-01
影响因子：
3
通讯作者：
Svergun, Dmitri I.
中科院分区：
文献类型：
--
作者：
Mertens, Haydyn D. T.;Svergun, Dmitri I.
X射线小角散射（SAXS）是一种用于溶液中生物大分子低分辨率结构表征的成熟方法。该技术提供了三维低分辨率的结构，使用从头算和刚体建模，并允许一个评估蛋白质和蛋白质复合物的寡聚状态。此外，SAXS是结构验证和柔性系统定量分析的强大工具，与X射线晶体学和NMR的高分辨率方法高度互补。目前，SAXS分析方法已达到先进的状态，允许自动化和快速表征蛋白质溶液的低分辨率模型，四级结构和低聚物组成。本文综述了利用小角X射线散射技术研究蛋白质和蛋白质复合物的主要方法。介绍了用于分析溶液中蛋白质的工具，并讨论了这些工具在现代结构生物学中所产生的影响。(C)2010年爱思唯尔公司All rights reserved.
Small-angle scattering of X-rays (SAXS) is an established method for the low-resolution structural characterization of biological macromolecules in solution. The technique provides three-dimensional low-resolution structures, using ab initio and rigid body modeling, and allow one to assess the oligomeric state of proteins and protein complexes. In addition, SAXS is a powerful tool for structure validation and the quantitative analysis of flexible systems, and is highly complementary to the high resolution methods of X-ray crystallography and NMR. At present, SAXS analysis methods have reached an advanced state, allowing for automated and rapid characterization of protein solutions in terms of low-resolution models, quaternary structure and oligomeric composition. In this communication, main approaches to the characterization of proteins and protein complexes using SAXS are reviewed. The tools for the analysis of proteins in solution are presented, and the impact that these tools have made in modern structural biology is discussed. (C) 2010 Elsevier Inc. All rights reserved.