Effect of recombinant platelet-derived growth factor (Regranex®) on wound closure in genetically diabetic mice
Effect of recombinant platelet-derived growth factor (Regranex®) on wound closure in genetically diabetic mice
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DOI：
10.1097/01.bcr.0000202898.11277.58
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发表时间：
2006-03-01
影响因子：
1.4
通讯作者：
Orgill, Dennis P.
中科院分区：
文献类型：
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作者：
Chan, Rodney K.;Liu, Perry H.;Orgill, Dennis P.
烧伤，特别是那些涉及大面积的烧伤，代表了复杂的伤口愈合问题。血小板衍生生长因子（PDGF）由活化的血小板释放以向伤口床募集炎性细胞。它具有促进血管生成和肉芽组织形成的作用。然而，局部PDGF对伤口闭合的有效性是可变的，范围从在猪模型中观察到的微小改善到在糖尿病小鼠模型中报告的显著改善。在这里，我们试图确定商业销售的PDGF-BB（Regranex（R））对遗传性糖尿病小鼠伤口闭合的有效性。将背上有1.5-cm（2）伤口的C57 BL/KsJ db+/db+小鼠及其宿主品系分成几组（每组n = 8），在受伤后连续5天局部施用Regranex（10 μ g/伤口）或赋形剂。使用计算机面积测量法分析伤口闭合率。肉芽组织的数量进行了组织学测定。我们的数据表明，糖尿病小鼠表现出显着延迟伤口闭合时，与他们的宿主菌株。局部应用Regranex（R）并没有改善伤口闭合的时间，但确实显著增加了肉芽组织的数量。我们目前使用市售的Regranex（R）的研究未能重现先前报道的发现，即PDGF改善了愈合受损的遗传性糖尿病小鼠的伤口闭合。
Burns, especially those involving large surface areas, represent a complex wound healing problem. Platelet-derived growth factor (PDGF) is released by activated platelets to recruit inflammatory cells toward the wound bed. It has effects on promoting angiogenesis and granulation tissue formation. However, the effectiveness of topical PDGF on wound closure is variable, ranging from little improvement observed in pig models to dramatic improvement reported in a diabetic mouse model. Here, we sought to determine the effectiveness of commercially sold PDGF-BB (Regranex (R)) on wound closure in genetically diabetic mice. C57BL/KsJ db+/db+ mice and its host strain bearing dorsal 1.5-cm(2) wounds were divided into groups (n = 8 in each group) receiving topical application of either Regranex (R) (10 mu g/wound) or vehicle for 5 consecutive days after wounding. The rate of wound closure was analyzed using computerized planimetry. The amount of granulation tissue was determined histologically. Our data indicate that diabetic mice exhibit a significant delay in wound closure when compared with their host strain. Topical application of Regranex (R) did not improve the time to wound closure but did significantly increase the amount of granulation tissue. Our current study using commercially available Regranex (R) failed to reproduce the previously reported finding that PDGF improved wound closure in healing impaired genetically diabetic mice.