Dendritic branching and homogenization of actin networks mediated by arp2/3 complex.
Dendritic branching and homogenization of actin networks mediated by arp2/3 complex.
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arp2/3 复合物介导的肌动蛋白网络的树突分支和均质化。
DOI：
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发表时间：
2004
影响因子：
8.6
通讯作者：
D. Wirtz
中科院分区：
文献类型：
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作者：
Yiider Tseng;D. Wirtz
运动细胞的细胞骨架利用辅助蛋白来局部调节其组织和微观力学。在这里，我们证明了Arp 2/3复合物的作用，独特的其他细胞骨架蛋白，肌动蛋白网络的“均质器”，促进非常迅速地形成均匀和僵硬的网络。对嵌入F-肌动蛋白网络中的微球的纳米追踪表明，Arp 2/3复合物促进了网络的形成，这些网络比对照网络显着更加均匀，这是一个协调弹性急剧爆发的独特特征。这些结果表明，Arp 2/3复合物具有稳定膜突起的独特功能，通过在爬行细胞的前缘形成均匀和刚性的肌动蛋白细胞骨架。
The cytoskeleton of motile cells exploits accessory proteins to locally modulate its organization and micromechanics. Here, we demonstrate that the Arp2/3 complex plays the role, unique among other cytoskeleton proteins, of an actin network "homogenizer," promoting the extremely rapid formation of homogeneous and stiff networks. Nanotracking of microspheres imbedded in F-actin networks reveals that the Arp2/3 complex promotes the formation of networks that are remarkably more homogeneous than control networks, a distinctive feature that coordinates a dramatic burst of elasticity. These results suggest that the Arp2/3 complex possesses a unique function of stabilizing membrane protrusions through the formation of homogeneous and stiff actin cytoskeleton at the leading edge of crawling cells.