A MOLECULAR MAP OF THE CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX - THE CLASS-II BETA-GENES ARE CLOSELY LINKED TO THE CLASS-I GENES AND THE NUCLEOLAR ORGANIZER
A MOLECULAR MAP OF THE CHICKEN MAJOR HISTOCOMPATIBILITY COMPLEX - THE CLASS-II BETA-GENES ARE CLOSELY LINKED TO THE CLASS-I GENES AND THE NUCLEOLAR ORGANIZER
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DOI：
10.1002/j.1460-2075.1988.tb03132.x
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发表时间：
1988-09-01
期刊：
影响因子：
11.4
通讯作者：
AUFFRAY, C
中科院分区：
文献类型：
--
作者：
GUILLEMOT, F;BILLAULT, A;AUFFRAY, C
我们在粘粒载体中克隆了覆盖320 kb鸡MHC（B复合体）的四个DNA簇，包括五个II类（B-L）β。定义两个相关同种型家族的基因。另外的B复合体基因已被发现使用组织特异性cDNA探针。粘粒片段已用于分离I类（B-F）转录物的cDNA。该转录本是迄今为止I类基因家族中最具分歧的已知成员，与存在于细胞中的6个B-F基因杂交。其中一个簇被证明含有两个rRNA转录单位的核仁组织者区（NOR），标记的端粒边界的B复合物。其它B复合体基因均不与哺乳动物II类MHC α杂交或具有其转录特征。或III类基因。我们已经获得的图表明，由于B-F和B-L β，B复合物不包含明确的I类和II类区域。基因与不相关的基因密切相关。此外，II类β. 基因在两个簇中与I类基因紧密相连，在第三个簇中与NOR紧密相连。
We have cloned in a cosmid vector four DNA clusters covering 320 kb of the chicken MHC (B complex), including five class II (B-L) .beta. genes defining two related isotypic families. Additional B complex genes have been revealed using tissue-specific cDNA probes. A cosmid fragment has been used to isolate a cDNA for a class I (B-F) transcript. This transcript, that is by far the most divergent known member of the class I gene family, hybridized to six B-F genes present in the cosmids. One of the clusters was shown to contain two rRNA transcriptional units from the nucleolar organizer region (NOR), marking the telomeric boundary of the B complex. None of the other B complex genes hybridizes to, or has the transcriptional characteristics of mammalian MHC class II .alpha. or class III genes. The map we have obtained shows that the B complex does not contain well defined class I and class II regions since B-F and B-L.beta. genes are closely associated with unrelated genes. Moreover, class II .beta. genes are very closely linked to class I genes in two clusters, and to the NOR in a third one.