Post-screening characterisation and in viva evaluation of an anti-inflammatory polysaccharide fraction from Eucommia ulmoides
Post-screening characterisation and in viva evaluation of an anti-inflammatory polysaccharide fraction from Eucommia ulmoides
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杜仲抗炎多糖组分的筛选后表征和体内评价
DOI：
10.1016/j.carbpol.2017.04.034
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发表时间：
2017-08-01
影响因子：
11.2
通讯作者：
Wang, Chunming
中科院分区：
文献类型：
--
作者：
Li, Qiu;Feng, Yanxian;Wang, Chunming
我们在此报告从杜仲中发现了一种具有抗炎活性的多糖，即 EUP1。我们根据酸度从这种草药中分离出三种多糖组分，并筛选它们在调节小鼠巨噬细胞表型方面的活性。其中，EUP1是唯一发挥这种功能的部分，它刺激Raw 264.7细胞表达CD206和关键的抗炎细胞因子白细胞介素10。通过一系列色谱和光谱分析充分表征了 EUP1，我们评估了其在体外和体内炎症模型中的抗炎作用。在脂多糖诱导的脓毒症小鼠模型中，给予EUP1可有效抑制主要炎症细胞因子的表达，减轻肺损伤并提高动物存活率。总之，EUP1 具有明确的化学结构和坚实的抗炎活性，可能成为进一步开发为抗菌治疗剂的有价值的候选者。 (C) 2017 Elsevier Ltd. 保留所有权利。
We report here the discovery of a polysaccharide, namely EUP1, with anti-inflammatory activity from the herb of Eucommia ulmoides Oliv. We separated three polysaccharide fractions from this herb based on acidity and screened them for their activity in modulating the phenotype of murine macrophages. Among them, EUP1 was the only fraction to exert such a function it stimulated Raw 264.7 cells to express CD206 and a key anti-inflammatory cytokine interleukin-10. Having fully characterised EUP1 with a series of chromatographic and spectroscopic analyses, we evaluated its anti-inflammatory effects in both in vitro and in vivo inflammatory models. In the murine model of sepsis induced by lipopolysaccharide, administration of EUP1 effectively suppressed the expression of major inflammatory cytokines, alleviated lung injury and increased animal survival rate. In summary, EUP1, with a clearly elucidated chemical structure and solid anti-inflammatory activity, may become a valuable candidate for further development into an anti-septic therapeutic agent. (C) 2017 Elsevier Ltd. All rights reserved.