Entrainment of the circadian clock in the liver by feeding
Entrainment of the circadian clock in the liver by feeding
复制标题
DOI：
10.1126/science.291.5503.490
复制
发表时间：
2001-01-19
期刊：
影响因子：
56.9
通讯作者：
Menaker, M
中科院分区：
文献类型：
--
作者：
Stokkan, KA;Yamazaki, S;Menaker, M
行为的昼夜节律是由大脑中与环境光周期耦合的振荡器驱动的。基因表达的昼夜节律广泛存在于外周器官中，目前尚不清楚这些多种节律如何耦合在一起形成一个连贯的系统。为了研究这种耦合，我们研究了食物供应周期（对行为产生强大的夹带效应）对肝、肺和视交叉上核（SCN）基因表达节律的影响。我们使用转基因大鼠模型，其组织在体外表达荧光素酶。虽然SCN的节律性仍然与明暗周期相锁定，但限制性进食迅速夹带肝脏，在2天内将其节律改变10小时。我们的研究结果表明，喂养周期可以独立于SCN和光周期来引导肝脏，并且他们建议需要重新检查哺乳动物的昼夜节律等级。他们还提出了一种可能性，即外周昼夜节律振荡器（如肝脏中的振荡器）可能主要通过节律行为（如进食）与SCN耦合。
Circadian rhythms of behavior are driven by oscillators in the brain that are coupled to the environmental light cycle. Circadian rhythms of gene expression occur widely in peripheral organs, It is unclear how these multiple rhythms are coupled together to form a coherent system. To study such coupling, we investigated the effects of cycles of food availability (which exert powerful entraining effects on behavior) on the rhythms of gene expression in the Liver, Lung, and suprachiasmatic nucleus (SCN). We used a transgenic rat model whose tissues express Luciferase in vitro. Although rhythmicity in the SCN remained phase-locked to the light-dark cycle, restricted feeding rapidly entrained the Liver, shifting its rhythm by 10 hours within 2 days. Our results demonstrate that feeding cycles can entrain the Liver independently of the SCN and the light cycle, and they suggest the need to reexamine the mammalian circadian hierarchy. They also raise the possibility that peripheral circadian oscillators Like those in the liver may be coupled to the SCN primarily through rhythmic behavior, such as feeding.