Electrochemical properties of 316L stainless steel with culturing L929 fibroblasts
Electrochemical properties of 316L stainless steel with culturing L929 fibroblasts
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DOI：
10.1098/rsif.2005.0108
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发表时间：
2006-08
影响因子：
3.9
通讯作者：
S. Hiromoto;T. Hanawa
中科院分区：
文献类型：
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作者：
S. Hiromoto;T. Hanawa
利用培养的小鼠成纤维细胞L929对316L不锈钢进行了动电位极化和阻抗试验，分别研究了L929细胞对316L钢的腐蚀行为以及316L钢与细胞之间的电化学界面。对316L钢进行I型胶原包被和培养L929细胞的电位阶试验，比较胶原和L929细胞的作用。316L钢的开路电位略有负向移动，无源电流密度随电池的增加而增加，表明钝化氧化膜的保护能力下降。点蚀电位随电池的增加而降低，表明抗点蚀能力降低。此外，阻抗测试中阴极电流密度的减小和扩散阻力参数的增大表明界面处的扩散系数减小。电位阶跃试验中阳极峰电流随细胞和胶原蛋白的增加而减小。因此，316L钢的耐蚀性随着L929的增加而降低。此外，胶原蛋白包被将提供一个类似于培养细胞的阳极反应环境。
Potentiodynamic polarization and impedance tests were carried out on 316L stainless steel with culturing murine fibroblast L929 cells to elucidate the corrosion behaviour of 316L steel with L929 cells and to understand the electrochemical interface between 316L steel and cells, respectively. Potential step test was carried out on 316L steel with type I collagen coating and culturing L929 cells to compare the effects of collagen and L929 cells. The open-circuit potential of 316L steel slightly shifted in a negative manner and passive current density increased with cells, indicating a decrease in the protective ability of passive oxide film. The pitting potential decreased with cells, indicating a decrease in the pitting corrosion resistance. In addition, a decrease in diffusivity at the interface was indicated from the decrease in the cathodic current density and the increase in the diffusion resistance parameter in the impedance test. The anodic peak current in the potential step test decreased with cells and collagen. Consequently, the corrosion resistance of 316L steel decreases with L929 cells. In addition, collagen coating would provide an environment for anodic reaction similar to that with culturing cells.