An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota.
An analysis of Pseudomonas genomic diversity in take-all infected wheat fields reveals the lasting impact of wheat cultivars on the soil microbiota.
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DOI：
10.1111/1462-2920.13038
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发表时间：
2015-11
影响因子：
5.1
通讯作者：
Malone JG
中科院分区：
文献类型：
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作者：
Mauchline TH;Chedom-Fotso D;Chandra G;Samuels T;Greenaway N;Backhaus A;McMillan V;Canning G;Powers SJ;Hammond-Kosack KE;Hirsch PR;Clark IM;Mehrabi Z;Roworth J;Burnell J;Malone JG
操纵与作物植物相关的土壤微生物群对控制作物病原体具有巨大的希望。然而，为了充分发挥这一潜力，我们需要更好地了解土壤环境与基因和表型之间的关系，这些基因和表型使微生物能够定植植物并有助于生物防治。最近2年，在全面真菌感染的情况下，对小麦品种对第二年作物产量的影响进行了调查，为在严格规定的田间条件下检查土壤微生物群提供了机会。第二年土壤样品的扩增子测序结果表明，假单胞菌受第一年种植的小麦品种的影响特别大。因此，分离出318株与根际相关的荧光假单胞菌菌株，并对其进行了多种遗传和表型特征的鉴定。同样，研究第一年种植的小麦品种对假单胞菌基因组多样性的程度和性质都施加了相当大的选择压力。此外，在假单胞菌群体的表型/遗传结构内，以及个体基因型与麦田外部环境之间，还发现了多重显著相关。本文概述的方法对我们理解植物与微生物的相互作用以及对复杂微生物群落的更广泛分析具有相当大的未来潜力。
Manipulation of the soil microbiota associated with crop plants has huge promise for the control of crop pathogens. However, to fully realize this potential we need a better understanding of the relationship between the soil environment and the genes and phenotypes that enable microbes to colonize plants and contribute to biocontrol. A recent 2 years of investigation into the effect of wheat variety on second year crop yield in the context of take‐all fungal infection presented the opportunity to examine soil microbiomes under closely defined field conditions. Amplicon sequencing of second year soil samples showed that P seudomonas spp. were particularly affected by the wheat cultivar grown in year one. Consequently, 318 rhizosphere‐associated P seudomonas fluorescens strains were isolated and characterized across a variety of genetic and phenotypic traits. Again, the wheat variety grown in the first year of the study was shown to exert considerable selective pressure on both the extent and nature of P seudomonas genomic diversity. Furthermore, multiple significant correlations were identified within the phenotypic/genetic structure of the Pseudomonas population, and between individual genotypes and the external wheat field environment. The approach outlined here has considerable future potential for our understanding of plant–microbe interactions, and for the broader analysis of complex microbial communities.