Insertion of an En/Spm-related transposable element into a floral homeotic gene DUPLICATED causes a double flower phenotype in the Japanese morning glory.
Insertion of an En/Spm-related transposable element into a floral homeotic gene DUPLICATED causes a double flower phenotype in the Japanese morning glory.
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DOI：
10.1046/j.1365-313x.2003.01896.x
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发表时间：
2003-11
期刊：
影响因子：
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通讯作者：
E. Nitasaka
中科院分区：
文献类型：
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作者：
E. Nitasaka
日本牵牛花花同源异型基因DUPLICATED（DP）的突变导致生殖器官被花被器官（花瓣和萼片）取代。该表型与转录因子的C功能MCM 1、AGAMOUS、DEFICIENS和SRF（MADS）-box基因家族的功能丧失表型相似。使用C功能MADS盒基因的共有序列分离DP。它的内含子-外显子结构在物种外非常保守，属于C功能MADS-box基因的FARINELLI（FAR）-pMADS 3亚类。在DP突变体中，一个增强子/抑制子-突变子（En/Spm）相关的转座因子，即牵牛属植物的转座因子（Tpn）-botan，被插入DP基因的第二内含子中，随后的切除事件导致原始Tpn元件和DP基因组的大部分缺失。一段Tpn 1相关的转座因子被确定在最近的插入位点的野生型DP基因组。该转座因子序列存在于所有供试的番薯属物种中。这一发现表明，插入事件起源于Ipomoea属的祖先物种。
Mutations in a floral homeotic gene DUPLICATED (DP) in the Japanese morning glory (Ipomoea nil) cause a substitution of reproductive organs to perianth organs (petals and sepals). This phenotype is similar to loss-of-function phenotypes of the C-function MCM1, AGAMOUS, DEFICIENS and SRF (MADS)-box gene family of transcription factors. DP was isolated using the consensus sequence of C-function MADS-box genes. Its intron-exon structure was well conserved beyond species, and it belongs to the FARINELLI (FAR)-pMADS3 subclass of C-function MADS-box genes. In a dp mutant, an Enhancer/Suppressor-mutator (En/Spm)-related transposable element, transposable element of Pharbitis nil (Tpn)-botan, was inserted in the second intron of DP gene, and the subsequent excision event led to a deletion of a substantial part of the original Tpn element and DP genome. A segment of Tpn1-related transposable element was identified at the recent insertion site of the wild-type DP genome. This transposable element sequence was present in all Ipomoea species tested. This finding suggests that the insertion event originated in an ancestral species of genus Ipomoea.