MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A
MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A
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DOI：
10.1016/j.gene.2016.12.030
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发表时间：
2017-03-20
期刊：
影响因子：
3.5
通讯作者：
Chen, Juan
中科院分区：
文献类型：
--
作者：
Chang, Cui;Liu, Te;Chen, Juan
DLK 1-DIO 3基因组印迹区域中的microRNA（miRNAs）簇包含几种在肿瘤增殖和侵袭中具有显著调节作用的miRNAs。这些miRNAs之一是miR-134- 3 p，其表达在人卵巢癌干细胞（OCSC）和CD 44-/CD 133-卵巢癌中显著变化。荧光素酶测定的结果显示，miR-134- 3 p通过与RAB 27 A mRNA的3 '-UTR结合而沉默RAB 27 A。miR-134- 3 p在人OCSC中的过表达不仅能抑制RAB 27 A的表达，还能有效下调一些肿瘤增殖和侵袭相关基因的表达。miR-134- 3 p的过表达不仅能抑制人卵巢癌干细胞的体外增殖和细胞周期进程，而且能降低其在裸鼠体内的致瘤性。(C)2016爱思唯尔B. V.保留所有权利。
The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44-/CD133 - ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3'-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes. Overexpression of miR-134-3p can not only inhibit the in vitro proliferation and cell cycle progression of human OCSCs but also can decrease the tumorigenicity in nude mice. (C) 2016 Elsevier B.V. All rights reserved.