Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography
Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography
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DOI：
10.1016/j.chroma.2004.09.005
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发表时间：
2004-10-15
影响因子：
4.1
通讯作者：
Chen, F
中科院分区：
文献类型：
--
作者：
Li, HB;Chen, F
天麻是我国著名的中药材，广泛用于治疗风湿、癫痫、瘫痪、偏瘫、腰痛、头痛、眩晕等症。首次采用高速逆流色谱法对天麻中的活性成分天麻素进行了制备性分离纯化。elata Blume.采用乙醇提取法从天麻中提取天麻素粗品。在超声处理下的elata Blume。以正丁醇-乙酸乙酯-水（2：3：5，v/v/v）为溶剂，采用高速逆流色谱法，从500 mg天麻素粗提物（10.3%）中分离出48 mg纯度为96%的天麻素，回收率约为90%。(C)2004 Elsevier B. V.保留所有权利。
Gastrodia elata Blume is a famous Chinese medicinal plant, which has been widely used for the treatment of rheumatism, epilepsy, paralysis, hemiplegia, lumbago, headache and vertigo. High-speed counter-current chromatography was successfully used for the first time for the preparative isolation and purification of the bioactive component gastrodin from G. elata Blume. The crude gastrodin was obtained by extraction with ethanol from the dried roots of G. elata Blume under sonication. Preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-butanol-ethyl acetate-water (2:3:5, v/v/v) was successfully performed yielding 48 mg gastrodin at 96% purity from 500 mg of the crude extract (10.3% gastrodin) with the recovery of approximately 90% in a one-step separation. (C) 2004 Elsevier B.V. All rights reserved.