Minichromosomes derived from the B chromosome of maize
Minichromosomes derived from the B chromosome of maize
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DOI：
10.1159/000082395
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发表时间：
2005-01-01
影响因子：
1.7
通讯作者：
Birchler, JA
中科院分区：
文献类型：
--
作者：
Kato, A;Zheng, YZ;Birchler, JA
本文描述了玉米B染色体上的14个微染色体。B染色体的着丝粒区域包含一个称为B重复序列的特异性重复DNA元件。该序列用于通过每代的Southern印迹分析确定不同类型的微型染色体在几代中的传递频率。一般来说，微型染色体的传递率低于单价染色体的理论50%频率。每个微型染色体的总体结构，确定使用荧光原位杂交（FISH）根尖染色体扩散。确定每个微型染色体的B着丝粒重复序列和相邻异染色质球序列的存在。在两种情况下，相对于祖先染色体，着丝粒球重复的量增加。其他分离株具有降低或不可检测水平的结序列。微型染色体的潜在用途进行了讨论。版权所有（C）2005 S. Karger AG，巴塞尔。
Fourteen minichromosomes derived from the B chromosome of maize are described. The centromeric region of the B chromosome contains a specific repetitive DNA element called the B repeat. This sequence was used to determine the transmission frequency of the different types of minichromosomes over several generations via Southern blot analysis at each generation. In general, the minichromosomes have transmission rates below the theoretical 50% frequency of a univalent chromosome. The gross structure of each minichromosome was determined using fluorescence in situ hybridization ( FISH) on root tip chromosome spreads. The presence of the B centromeric repeat and of the adjacent heterochromatic knob sequences was determined for each minichromosome. In two cases, the amount of the centromeric knob repeat is increased relative to the progenitor chromosome. Other isolates have reduced or undetectable levels of the knob sequence. Potential uses of the minichromosomes are discussed. Copyright (C) 2005 S. Karger AG, Basel.