Detection of Campylobacter and Escherichia coli O157:H7 from filth flies by polymerase chain reaction
Detection of Campylobacter and Escherichia coli O157:H7 from filth flies by polymerase chain reaction
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DOI：
10.1111/j.0269-283x.2004.00502.x
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发表时间：
2004-09-01
影响因子：
1.9
通讯作者：
Steelman, CD
中科院分区：
文献类型：
--
作者：
Szalanski, AL;Owens, CB;Steelman, CD
在粪便和其他有机垃圾（污蝇）中繁殖的苍蝇（双翅目：蝇科）被认为是病原菌的载体，包括引起人类出血性结肠炎的大肠杆菌 O157:H7 和人类肠炎的主要病原体弯曲杆菌。通过检查弯曲杆菌属的流行情况，研究了污蝇在美国这些病原体流行病学中的潜在作用。来自阿肯色州两个火鸡设施的大肠杆菌 O157:H7。使用大肠杆菌 H7、O157 和弯曲杆菌属特异性引物，对家蝇、Stomoxys calcitrans (Linnaeus)、Hydrotaea aenescens (Wiedemann)、Adia cinerella Fallen 和火鸡粪便样本的 DNA 提取进行聚合酶链反应。培养证实果蝇携带活的弯曲杆菌属。和大肠杆菌 O157:H7。这项研究的结果表明，北美家禽设施中的 M. Domestica、S. calcitrans、H. aenescens 和 Anthomylds 能够携带弯曲杆菌，而大肠杆菌 O157:H7 则由与家禽相关的家蝇和黑粪蝇携带。该PCR方法为鉴定弯曲杆菌属提供了一种快速有效的方法。和直接来自单个污蝇的大肠杆菌 O157:H7。
Flies (Diptera: Muscidae) that breed in faeces and other organic refuse (filth flies) have been implicated as vectors of pathogenic bacteria including Escherichia coli O157:H7, which cause haemorrhagic colitis in humans, and Campylobacter, which is the principal causative agent of human enteritis. The potential role of filth flies in the epidemiology of these pathogens in the United States was investigated by examining the prevalence of Campylobacter spp. and E. coli O157:H7 from two Arkansas turkey facilities. Polymerase chain reaction was conducted on DNA extractions of individual Musca domestica Linnaeus, Stomoxys calcitrans (Linnaeus), Hydrotaea aenescens (Wiedemann), Adia cinerella Fallen and turkey faecal samples using primers specific for E. coli H7, O157 and Campylobacter spp. Culturing verified that the flies were carrying viable Campylobacter spp. and E. coli O157:H7. Results from this Study indicated that M. domestica, S. calcitrans, H. aenescens and Anthomylds are capable of carrying Campylobacter in North American poultry facilities and that the E. coli O157:H7 is carried by house flies and black dump flies associated with Poultry. This PCR method provided a rapid and effective method to identify Campylobacter spp. and E. coli O157:H7 directly from individual filth flies.