Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis
Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis
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CCN2/CTGF在膜内成骨中的趋化特性分析
DOI：
10.1007/978-1-0716-2744-0_16
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Takano-Yamamoto T.
中科院分区：
文献类型：
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作者：
Takeshita N;Takano-Yamamoto T.
趋化性是细胞响应于称为趋化因子的细胞外分子的梯度而定向迁移。骨的发育、生长、重塑和骨折愈合通过膜内成骨来推进。在膜内成骨的早期阶段，前成骨细胞对未来骨形成位点的趋化性是正常骨形成的关键细胞过程。然而，前成骨细胞趋化性的分子生物学机制尚未完全清楚。最近，我们首次阐明了细胞通讯网络因子2（CCN 2）/结缔组织生长因子（CTGF）-整合素α5-Ras轴在膜内成骨新骨形成过程中对前成骨细胞趋化性的关键作用。在本章中，我们详细描述的程序，在体内和体外试验，以调查CCN 2/CTGF的趋化特性和膜内成骨过程中的分子生物学机制。
Chemotaxis is a directed migration of cells in response to a gradient of extracellular molecules called chemoattractants. Development, growth, remodeling, and fracture healing of bones are advanced through intramembranous osteogenesis. Chemotaxis of preosteoblasts toward future bone formation sites observed in the early stage of intramembranous osteogenesis is a critical cellular process for normal bone formation. However, molecular biological mechanisms of the chemotaxis of preosteoblasts are not fully understood. We have recently clarified, for the first time, the critical role of the cellular communication network factor 2 (CCN2)/connective tissue growth factor (CTGF)-integrin α5-Ras axis for chemotaxis of preosteoblasts during new bone formation through intramembranous osteogenesis. In this chapter, we describe in detail the procedures of the in vivo and in vitro assays to investigate the chemotactic property of CCN2/CTGF and its underlying molecular biological mechanisms during intramembranous osteogenesis.