Distribution and change of collagen types I and III and elastin in developing leg muscle in rat.
Distribution and change of collagen types I and III and elastin in developing leg muscle in rat.
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发表时间：
2006-09
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通讯作者：
T. Kurose;Y. Asai;Emiko Mori;Daisuke Daitoku;S. Kawamata
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作者：
T. Kurose;Y. Asai;Emiko Mori;Daisuke Daitoku;S. Kawamata
用免疫组织化学方法研究了I型、III型胶原和弹性蛋白在大鼠腿部肌肉发育过程中的分布。从0日龄到8周龄，腓肠肌和足底肌的大小逐渐增大。肌肉结缔组织的发育顺序为肌外膜、肌外膜、肌内膜。新生儿肌上膜含有相当数量的I型和III型胶原和一些弹性蛋白。外膜中的这些成分在发育过程中的分布几乎保持不变。0d时肌束膜几乎没有I型和III型胶原或弹性蛋白。I型胶原和弹性蛋白分别在2周和1周时略有增加，并恢复到以前的水平。然而，III型胶原在1周后增加并变得丰富。在肌内膜中，I型胶原和弹性蛋白在出生后的生长过程中含量很少，而III型胶原在出生后2周后逐渐增加。肌内肌腱始终表现出I型胶原的强烈反应和弹性蛋白的弱染色，而III型胶原的染色在1周后减少，最后局限于肌肉内肌腱的表面。这项研究清楚地表明，胶原蛋白的分布在发育过程中发生了显著的变化，但弹性蛋白的分布没有变化。肌束膜和肌内膜中III型胶原的增加和肌内肌腱中III型胶原的减少可能反映了对这些结缔组织施加的功能需求，即前两者的剪切力和后一种结缔组织的拉伸负荷。
The distribution of collagen types I and III and elastin in the developing leg muscles were studied by immunohistochemistry in rat. From 0-day to 8-weeks old, the size of the gastrocnemius and plantaris muscles increased. The muscle connective tissue developed in the order of epimysium, perimysium and finally endomysium. The epimysium contained a considerable amount of collagen types I and III and some elastin in the neonates. These components in the epimysium remained almost unchanged in their distribution during development. The perimysium had little collagen type I and III or elastin at 0 day. Collagen type I and elastin slightly increased around 2 and 1 week, respectively, and returned to the previous levels. Collagen type III, however, increased and became abundant after 1 week. In the endomysium, the amounts of collagen type I and elastin were slight during postnatal growth, while collagen type III gradually increased after 2 weeks. The intramuscular tendons consistently showed intense reactivity for collagen type I and weak staining for elastin, whereas the staining for collagen type III decreased after 1 week and was finally restricted to the surface of intramuscular tendons. This study clearly demonstrated that the distribution of collagens, but not of elastin, significantly changed during development. The increase in collagen type III in the perimysium and endomysium, and its decrease in the intramuscular tendons probably reflect functional demands imposed on these connective tissues, i.e., shear forces in the former two and tensile loading in the latter.