Antibodies to human heat-shock protein 60 are associated with the presence and severity of coronary artery disease: evidence for an autoimmune component of atherogenesis.
Antibodies to human heat-shock protein 60 are associated with the presence and severity of coronary artery disease: evidence for an autoimmune component of atherogenesis.
复制标题
人热休克蛋白 60 的抗体与冠状动脉疾病的存在和严重程度相关：动脉粥样硬化形成的自身免疫成分的证据。
DOI：
10.1161/01.cir.103.8.1071
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发表时间：
2001
期刊：
影响因子：
37.8
通讯作者：
Epstein,SE
中科院分区：
文献类型：
--
作者：
Zhu,J;Quyyumi,AA;Rott,D;Csako,G;Wu,H;Halcox,J;Epstein,SE
背景--据报道，分枝杆菌热休克蛋白(HSP)65的抗体与颈动脉增厚有关。方法和结果：对391例(62%男性，平均年龄57岁)经冠状动脉造影术确诊为冠心病的患者的血液样本，用酶联免疫吸附试验检测抗人HSP60抗体。我们发现，75%的研究对象有抗HSP60抗体。血清阳性患者冠心病患病率高于阴性患者(68%比49%，P=0.0009)。冠心病组HSP60抗体平均滴度高于非冠心病组(P=0.008)。未发现HSP60抗体与感染或炎症之间的关联。重要的是，HSP60抗体与疾病严重程度有关。1支、2支和3支冠脉病变患者热休克蛋白60抗体阳性率分别为76%、80%和85%，而非冠心病组为%(P趋势=0.003)。抗体滴度的升高与病变血管的数量之间也发现了类似的关联(P=0.03)。在根据年龄、种族、性别、吸烟、糖尿病、高胆固醇血症、高血压和C反应蛋白水平对传统危险因素进行调整后，HSP60抗体与冠心病严重程度之间的显著相关性仍然存在。血管病变数目的调整OR为1.86(95%可信区间为1.13至3.04)。结论：这是第一项显示人类HSP60抗体与冠心病的存在和严重程度显著相关的研究。
Background—Antibodies to mycobacterial heat-shock protein (HSP) 65 have been reported to be associated with carotid artery thickening. We examined whether antibodies to human HSP60 are associated with the risk of coronary artery disease (CAD).Methods and Results—Blood samples from 391 patients (62% men, mean age 57 years) being evaluated for CAD by coronary angiography were tested for IgG antibodies to human HSP60 by ELISA. We found that 75% of the study subjects had anti-HSP60 antibodies. The prevalence of CAD was increased in seropositive compared with seronegative patients (68% versus 49%,P=0.0009). Mean titers of HSP60 antibodies were higher in CAD patients than in non-CAD patients (P=0.008). No association between HSP60 antibodies and infection or inflammation was found. Importantly, HSP60 antibodies were related to disease severity. The prevalence of HSP60 antibodies was 76%, 80%, and 85% in patients with 1-, 2-, and 3-vessel disease, compared with 64% in patients without CAD (Pfor trend=0.003). A similar association between increasing antibody titers and number of diseased vessels was also found (P=0.03). Significant associations between antibodies to HSP60 and CAD severity persisted after adjustment for traditional risk factors by age, race, sex, smoking, diabetes, hypercholesterolemia, hypertension, and C-reactive protein levels. Adjusted OR for number of vessels diseased was 1.86 (95% CI 1.13 to 3.04).Conclusions—This is the first study demonstrating a significant association between human HSP60 antibodies and both the presence and severity of CAD.