Differential chemokine expression in tissues involved by Hodgkin's disease: Direct correlation of eotaxin expression and tissue eosinophilia
Differential chemokine expression in tissues involved by Hodgkin's disease: Direct correlation of eotaxin expression and tissue eosinophilia
复制标题
DOI：
10.1182/blood.v93.8.2463.408k02_2463_2470
复制
发表时间：
1999-04-15
期刊：
影响因子：
20.3
通讯作者：
Tosato, G
中科院分区：
文献类型：
--
作者：
Teruya-Feldstein, J;Jaffe, ES;Tosato, G
何杰金氏病（HD）是一种淋巴系统恶性肿瘤，其特征是罕见的恶性细胞被大量的炎性细胞包围。在这项研究中，我们研究了趋化因子在不同亚型HD中对炎症细胞募集的潜在贡献。趋化因子是作为化学引诱剂和细胞活化调节剂而具有活性的小蛋白质。我们发现HD组织通常表达较高水平的干扰素-γ-诱导蛋白-10（IP-10）、Mig. RANTES，巨噬细胞炎性蛋白-1 α（MIP-1 α）。和嗜酸性粒细胞趋化因子，而不是巨噬细胞衍生的趋化因子（MDC），比淋巴组织增生（LH）。在HD亚型中，IP-10和Mig的表达在混合细胞性（MC）亚型中最高，而eotaxin和MDC的表达在结节性硬化（NS）亚型中最高。在肿瘤细胞中EB病毒（EBV）感染的证据与IP-10、RANTES和MIP-1 α的表达水平之间检测到显著的直接相关性。嗜酸性粒细胞趋化因子的表达水平与组织嗜酸性粒细胞增多的程度直接相关。通过免疫组织化学，IP-10，Mig和eotaxin蛋白定位于恶性Reed-Sternberg（RS）细胞及其变体，以及周围的一些炎症细胞。在血管的成纤维细胞和平滑肌细胞中也检测到Eotaxin。这些结果提供了高水平的趋化因子在HD组织中表达的证据，并表明趋化因子可能在炎症细胞浸润到HD所涉及的组织中的募集中发挥重要作用。(C)1999年，美国血液学会。
Hodgkin's disease (HD) is a lymphoid malignancy characterized by infrequent malignant cells surrounded by abundant inflammatory cells. In this study, we examined the potential contribution of chemokines to inflammatory cell recruitment in different subtypes of HD. Chemokines are small proteins that are active as chemoattractants and regulators of cell activation. We found that HD tissues generally express higher levels of interferon-gamma-inducible protein-10 (IP-10), Mig. RANTES, macrophage inflammatory protein-1 alpha (MIP-1 alpha). and eotaxin, but not macrophage-derived chemotactic factor (MDC), than tissues from lymphoid hyperplasia (LH). Within HD subtypes, expression of IP-10 and Mig was highest in the mixed cellularity (MC) subtype, whereas expression of eotaxin and MDC was highest in the nodular sclerosis (NS) subtype. A significant direct correlation was detected between evidence of Epstein-Barr virus (EBV) infection in the neoplastic cells and levels of expression of IP-10, RANTES, and MIP-1 alpha. Levels of eotaxin expression correlated directly with the extent of tissue eosinophilia. By immunohistochemistry, IP-10, Mig, and eotaxin proteins localized in the malignant Reed-Sternberg (RS) cells and their variants, and to some surrounding inflammatory cells. Eotaxin was also detected in fibroblasts and smooth muscle cells of vessels. These results provide evidence of high level chemokine expression in HD tissues and suggest that chemokines may play an important role in the recruitment of inflammatory cell infiltrates into tissues involved by HD. (C) 1999 by The American Society of Hematology.