Optimum extraction conditions for arbutin from Asian pear peel by supercritical fluid extraction (SFE) using Box-Behnken design
Optimum extraction conditions for arbutin from Asian pear peel by supercritical fluid extraction (SFE) using Box-Behnken design
复制标题
采用 Box-Behnken 设计超临界流体萃取 (SFE) 从亚洲梨皮中提取熊果苷的最佳条件
DOI：
10.5897/jmpr11.890
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发表时间：
2012
影响因子：
--
通讯作者：
J. Eun
中科院分区：
文献类型：
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作者：
Byung;J. Eun
亚洲梨（Pyrus pyrifolia cv.果汁加工过程中产生的副产品，是熊果苷的良好来源，熊果苷是一种多酚，对皮肤有美白作用。采用Box-Behnken试验设计，采用响应面法优化超临界流体萃取亚洲梨果皮中熊果苷的工艺条件。熊果苷采用甲醇、乙醇共溶剂提取。进行三水平四因素Box-Behnken实验设计，以评价四个独立变量（共溶剂浓度（22 - 30%）、提取压力（250 - 300 bar）、提取温度（30 - 60 ℃）和提取时间（30 - 60 min））的组合效应，分别编码为X1、X2、X3和X4。响应面回归方程的决定系数（R2）为0.89（p<0.01），甲醇作为助溶剂，乙醇为0.84（p<0.01）。在26%、275 bar、45°C和45 min条件下，甲醇超临界流体萃取的熊果苷含量最高，为3.35 mg/g。总之，在26%甲醇作为助溶剂、萃取压力为275 bar、萃取温度为45°C和萃取时间为45 min的超临界流体萃取条件下，亚洲梨果皮中熊果苷的萃取效率最高。
关键词：亚洲梨，熊果苷，响应面法，超临界流体萃取，Box-Behnken试验设计。
Asian pear (Pyrus pyrifolia cv. Niitaka) peel, a by-product that results from juice processing, is a good source of arbutin, a polyphenol, which has a whitening effect on the skin. The objective of this study was to investigate the optimum extraction conditions for arbutin from Asian pear peel by supercritical fluid extraction (SFE) using response surface methodology (RSM) based on Box-Behnken experiment design. Arbutin was extracted using co-solvent, methanol and ethanol. A three-level four-factor Box-Behnken experiment design was performed to evaluate the combination effect of four independent variances, co-solvent concentration (22 to 30%), extraction pressure (250 to 300 bar), extraction temperature (30 to 60°C) and extraction time (30 to 60 min), coded for X1, X2, X3 and X4, respectively. The coefficients of determination (R2) of response surface regression equations were 0.89 (p<0.01) for methanol as a co-solvent and 0.84 (p<0.01) for ethanol. Arbutin content by SFE with methanol was the highest with 3.35 mg/g at 26%, 275 bar, 45°C and 45 min. In conclusion, arbutin from Asian pear peel would be extracted from the most efficiently combination of 26% methanol as co-solvent, extraction pressure of 275 bar, extraction temperature of 45°C and extraction time of 45 min in the SFE.
Key words: Asian pear, arbutin, response surface methodology, supercritical fluid extraction, Box-Behnken experiment design.