An Update on the Classification of Kentucky Bluegrass Cultivars and Accessions Based on Microsatellite (SSR) Markers
An Update on the Classification of Kentucky Bluegrass Cultivars and Accessions Based on Microsatellite (SSR) Markers
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DOI：
10.2135/cropsci2017.11.0689
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发表时间：
2018-07-01
期刊：
影响因子：
2.3
通讯作者：
Meyer, William A.
中科院分区：
文献类型：
--
作者：
Honig, Josh A.;Averello, Vincenzo;Meyer, William A.
肯塔基州早熟禾（Poa pratensis L.）是一种重要的多年生牧草和舒适草坪草物种，在整个北美和欧洲的温带地区使用。肯塔基州早熟禾栽培品种和种质表现出广泛的倍性水平、形态性状、草坪草质量特性以及对生物和非生物胁迫的耐受性。因此，栽培品种分类系统已经被开发用于基于系谱、草坪性能、形态性状和遗传相关性（DNA标记）对肯塔基州早熟禾栽培品种和种质进行分类。本研究的目的是利用简单重复序列（SSR）标记评估肯塔基州早熟禾品种间和品种内的遗传多样性，并在肯塔基州早熟禾分类系统中对新种质进行分类。本研究利用21个SSR标记对144份早熟禾品种和材料（共1152份样品）的8个个体进行了基因分型。SSR标记成功地将所有条目分为不同的分类组，并为部分修订以前的DNA标记为基础的分类系统提供了理由。大多数品种和加入唯一确定与目前的SSR标记，和SSR标记为基础的遗传关系的分类类型内的个人表现出与育种历史记录的协议。将60多个新的肯塔基州早熟禾国家草坪草评价计划条目放入更新的分类组中，本研究中使用的SSR标记可用于将来对新品种和新材料进行基因分型和分配到肯塔基州早熟禾分类类型中，并评估条目之间的遗传相关性。
Kentucky bluegrass (Poa pratensis L.) is an important perennial forage and amenity turfgrass species used throughout temperate regions in North America and Europe. Kentucky bluegrass cultivars and accessions exhibit a wide range of ploidy levels, morphological traits, turfgrass quality characteristics, and tolerances to both biotic and abiotic stresses. As such, cultivar classification systems have been developed to categorize Kentucky bluegrass cultivars and accessions on the basis of pedigree, turf performance, morphological traits, and genetic relatedness (DNA markers). The objectives of the current study were to assess the genetic diversity among and within Kentucky bluegrass entries using simple sequence repeat (SSR) markers, and to categorize new germplasm in the Kentucky bluegrass classification system. In this study, 21 SSR markers were used to genotype eight individuals from each of 144 bluegrass cultivars and accessions (1152 total samples). The SSR markers successfully categorized all entries into distinct classification groups and provided justification for a partial revision to the previous DNA marker-based classification system. The majority of cultivars and accessions were uniquely identified with the current set of SSR markers, and SSR marker-based genetic relationships of individuals within classification types showed agreement with breeding history records. Over 60 new Kentucky bluegrass National Turfgrass Evaluation Program entries were placed into updated classification groups, and the SSR markers used in this study can be used in the future to genotype and assign new cultivars and accessions into Kentucky bluegrass classification types and assess the genetic relatedness among entries.