Longitudinal Change Detected by Spectral Domain Optical Coherence Tomography in the Optic Nerve Head and Peripapillary Retina in Experimental Glaucoma
Longitudinal Change Detected by Spectral Domain Optical Coherence Tomography in the Optic Nerve Head and Peripapillary Retina in Experimental Glaucoma
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DOI：
10.1167/iovs.10-5599
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发表时间：
2011-03-01
影响因子：
4.4
通讯作者：
Burgoyne, Claude F.
中科院分区：
文献类型：
--
作者：
Strouthidis, Nicholas G.;Fortune, Brad;Burgoyne, Claude F.
目的.研究在实验性青光眼（EG）中通过光谱域光学相干断层扫描（SDOCT）是否可检测到视神经乳头（ONH）深处的纵向变化，以及在海德堡视网膜断层扫描（HRT; Heidelberg Engineering，Heidelberg，德国）定义的表面形貌凹陷开始时是否可检测到这些变化。每1至3周在9只恒河猴的双眼中进行纵向SDOCT成像（Spectralis; Heidelberg Engineering）。每只眼睛中有一只眼睛接受小梁激光诱导的IOP升高。4名盲法操作者使用自定义软件描绘内界膜（ILM）、视网膜神经纤维层（RNFL）、Bruch膜/视网膜色素上皮（BM/RPE）、神经管开口（NCO）和前筛板表面（ALCS）。在HRT检测到的表面凹陷开始时（随访1; [HA]）和最近的图像（随访2; [FU 2]），评估并比较EG和对照（非激光）眼之间的纵向变化。FU 1时，EG眼的平均IOP为7.1 - 24.6 mm Hg，FU 2时为13.5 - 31.9 mm Hg。在对照眼中，平均IOP为7.2至12.6 mm Hg（FLU）和8.9至16.0 μ m Hg（FU 2）。在神经视网膜边缘处，ALCS深度显著增加（配对t检验，P < 0.01），而RNFL厚度无变化。然而，在FU 2时，显著的椎板前组织变薄、NCO后移位和RNEL变薄。纵向SDOCT成像可以检测EG眼中的深ONH变化，其中最早的是在HRT检测的ONH表面高度降低时出现。这些参数代表了SDOCT检测人类受试者脑水肿进展的现实目标。（Invest Ophthalmol维斯Se 2011;52：1206-1219）DOI：10.1167/iovs.10-5599
PURPOSE. To investigate whether longitudinal changes deep within the optic nerve head (ONH) are detectable by spectral domain optical coherence tomography (SDOCT) in experimental glaucoma (EG) and whether these changes are detectable at the onset of Heidelberg Retina Tomography (HRT; Heidelberg Engineering, Heidelberg, Germany)-defined surface topography depression.METHODS. Longitudinal SDOCT imaging (Spectralis; Heidelberg Engineering) was performed in both eyes of nine rhesus macaques every 1 to 3 weeks. One eye of each underwent trabecular laser-induced IOP elevation. Four masked operators delineated internal limiting membrane (ILM), retinal nerve fiber layer (RNFL), Bruch's membrane/retinal pigment epithelium (BM/RPE), neural canal opening (NCO), and anterior lamina cribrosa surface (ALCS) by using custom software. Longitudinal changes were assessed and compared between the EG and control (nonlasered) eyes at the onset of HRT-detected surface depression (follow-up 1; [HA]) and at the most recent image (follow-up 2; [FU2]).RESULTS. Mean IOP in EG eyes was 7.1 to 24.6 nun Hg at FU1 and 13.5 to 31.9 nun Hg at FU2. In control eyes, the mean IOP was 7.2 to 12.6 mm Hg (FLU) and 8.9 to 16.0 'um Hg (FU2). At neuroretinal rim decreased and ALCS depth increased significantly (paired t-test, P < 0.01); no change in RNFL thickness was detected. At FU2, however, significant prelaminar tissue thinning, posterior displacement of NCO, and RNEL thinning were observed.CONCLUSION. Longitudinal SDOCT imaging can detect deep ONH changes in EG eyes, the earliest of which are present at the onset of HRT-detected ONH surface height depression. These parameters represent realistic targets for SDOCT detection of glaucomatous progression in human subjects. (Invest Ophthalmol Vis Se 2011;52:1206-1219) DOI:10.1167/iovs.10-5599