Immunohistochemical localization of ubiquitin cross-reactive protein in human tissues.
Immunohistochemical localization of ubiquitin cross-reactive protein in human tissues.
复制标题
人体组织中泛素交叉反应蛋白的免疫组织化学定位。
DOI：
10.1002/path.1711770210
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Mayer,RJ
中科院分区：
文献类型：
--
作者：
Lowe,J;McDermott,H;Loeb,K;Landon,M;Haas,AL;Mayer,RJ
泛素交叉反应蛋白（UCRP）是一种干扰素诱导型泛素同系物，组成型存在于细胞中，可以与其他蛋白质缀合。使用特征化的 UCRP 多克隆抗血清，对石蜡处理的正常人体组织和已知含有泛素化细胞内包涵体的人体组织进行 UCRP 的免疫组织化学定位。 UCRP 抗体对淋巴细胞、横纹肌和平滑肌、多种上皮细胞和神经元进行免疫染色。组织之间的染色水平差异很大，但呈一致的点状图案。神经肌肉接头和条纹的定位与泛素蛋白缀合物抗血清的描述类似。 UCRP 抗体未检测到以抗泛素免疫反应为特征的包涵体。重要的是，由于 UCRP 也可以通过缀合泛素的抗血清检测到，因此未来对组织切片中泛素分布的研究现在必须考虑与 UCRP 可能的交叉反应性。
Ubiquitin cross‐reactive protein (UCRP) is an interferon‐inducible ubiquitin homologue which is constitutively present in cells and can be conjugated to other proteins. Using a characterized polyclonal antiserum to UCRP, immunohistochemical localization of UCRP was performed on paraffin‐processed normal human tissues and in human tissues known to contain ubiquitinated intracellular inclusions. The antibody to UCRP immunostained lymphoid cells, striated and smooth muscle, several epithelia, and neurons. The level of staining varied greatly between tissues but was in a consistent punctate pattern. Localization to neuromuscular junctions and striations is similar to that described for antisera to ubiquitin‐protein conjugates. Inclusion bodies characterized by immunoreactivity to anti‐ubiquitin were not detected by the antibody to UCRP. Importantly, because UCRP may also be detected by antisera to conjugated ubiquitin, future studies on the distribution of ubiquitin in tissue sections must now take account of possible cross‐reactivity with UCRP.