Restructured fungal community diversity and biological interactions promote metolachlor biodegradation in soil microbial fuel cells
Restructured fungal community diversity and biological interactions promote metolachlor biodegradation in soil microbial fuel cells
复制标题
重组真菌群落多样性和生物相互作用促进土壤微生物燃料电池中异丙甲草胺的生物降解
DOI：
10.1016/j.chemosphere.2019.01.040
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Li Yongtao
中科院分区：
文献类型：
--
作者：
Li Xiaojing;Li Yue;Zhao Xiaodong;Zhang Xiaolin;Zhao Qian;Wang Xin;Li Yongtao
土壤微生物燃料电池（MFCs）为去除异丙甲草胺提供了一个用之不竭的电子受体。在23 d后，引入电极后，异丙草胺的降解率提高了33% ~ 36%。在封闭的mfc中，最优势属mortiellae的丰度从原始土壤的17%增加到43% ~ 54%，而aphanascu和penicillium的丰度分别从14% ~ 11%下降到0.24% ~ 0.39%和0.38 ~ 0.72%。此外，观察到独特的otu扩增10倍，主要来自电极表面的增加。不同处理的样品聚集在一起，特别是靠近阴极的样品。线性判别分析表明，在原始土壤和处理过的土壤之间存在着明显的生物标记。共生网络表明，mortierella普遍与共存的物种竞争生长，而ladosporium则与其他36个属的物种表现出最多的亲缘关系。相关分析表明，与降解相关的菌种为toMortierella、Kernia、ChaetomiumandTrichosporon，与产电相关的菌种为debaryomyces hanseniii和mortierella polycephala。重要的是，这项研究首次揭示了具有降解污染物和发电功能的土壤mfc中的真菌群落结构。
Soil microbial fuel cells (MFCs) provide an inexhaustible electron acceptor for the removal of metolachlor andin situbiocurrent stimulation for fungal activity was investigated. The metolachlor degradation rates enhanced by 33%–36% upon the introduction of electrodes after 23 d. In closed MFCs, the abundance ofMortierellaas the most dominant genus increased to 43%–54% from 17% in the original soil, whereas those ofAphanoascusandPenicilliumdecreased to 0.24%–0.39% and 0.38–0.72% from 14% to 11%, respectively. Additionally, a 10-fold amplification of unique OTUs was observed, mainly from increase on the electrode surface. The different treatments were clustered, especially samples near the cathode. The linear discriminant analysis showed thatAphanoascus fulvescensacted as a biomarker between the original and treated soils. The co-occurrence networks demonstrated thatMortierellauniversally competed for growth with coexisting species whileCladosporiumexhibited the most affiliations with species from the 36 other genera present. The correlation analysis indicated that the species associated with degradation belonged toMortierella,Kernia,ChaetomiumandTrichosporon, while the species associated with electrogenesis wereDebaryomyces hanseniiandMortierella polycephala. Importantly, this study is the first to reveal fungal community structure in soil MFCs with degrading pollutants and producing electricity.