The overexpression of insect endogenous small RNAs in transgenic rice inhibits growth and delays pupation of striped stem borer (Chilo suppressalis)
The overexpression of insect endogenous small RNAs in transgenic rice inhibits growth and delays pupation of striped stem borer (Chilo suppressalis)
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DOI：
10.1002/ps.4477
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发表时间：
2017-07-01
影响因子：
4.1
通讯作者：
Chen, Hao
中科院分区：
文献类型：
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作者：
Jiang, Shan;Wu, Hao;Chen, Hao
背景：二化螟是世界性的水稻害虫。RNA干扰（RNA interference，RNAi）已成为培育抗虫作物的一种有效方法。在前期的研究中，我们在水稻中过表达了5个靶向SSB管家基因的双链RNA（dsRNA），但获得的转dsRNA水稻植株中没有一个表现出对SSB的显著影响。结果：利用人工miRNA（amiRNA）表达技术，筛选出13个SSB内源小RNA，预测为SSB新型microRNA（miRNAs）。摄食试验表明，在13种筛选出的SSB novelmiRNAs中，有2种对转基因水稻表达的SSB幼虫的摄食有显著的生长抑制作用。当SSB幼虫连续饲喂表达SSB novelmiRNA候选物csu-novel-miR 15的转基因水稻（csu-15水稻）时，化蛹延迟4天。基因表达分析证实，至少六个SSB单基因的表达水平显著改变（即，结论：我们的研究证明了一种利用SSB内源小RNA开发RNAi作物用于害虫管理的新策略;该策略不同于针对某些昆虫内源基因的转基因dsRNA或amiRNA导致的常见RNAi。(C)2016年化学工业协会
BACKGROUND: The striped stem borer (SSB), Chilo suppressalis Walker, is a major rice insect pest worldwide. RNA interference (RNAi) has become a promising strategy for developing insect-resistant crops. In a previous study, five double-stranded RNAs (dsRNAs) targeting important SSB housekeeping genes were overexpressed in rice, but none of the acquired dsRNA-transgenic rice plants showed significant effects on SSB.RESULTS: Thirteen selected SSB endogenous small RNAs, predicted as SSB novel microRNAs (miRNAs), were overexpressed in rice using artificial miRNA (amiRNA) expression technology. Feeding tests showed that two out of 13 selected SSB novelmiRNAs caused significant growth inhibition for feeding SSB larvae based on transgenic rice expression. Pupation was delayed 4 days when SSB larvae consecutively fed on transgenic rice expressing the SSB novelmiRNA candidate csu-novel-miR15 (csu-15 rice). Gene expression analysis confirmed that the expression levels of at least six SSB unigenes significantly changed (i.e., were upor down-regulated) after feeding on csu-15 rice.CONCLUSIONS: Our research demonstrated a novel RNAi strategy using SSB endogenous small RNAs to develop RNAi crops for pest management; this strategy is different from the common RNAi resulting from transgenic dsRNAs or amiRNAs targeting certain insect endogenous genes. (C) 2016 Society of Chemical Industry