Molecular nature of the Drosophila sex determination signal and its link to neurogenesis.
Molecular nature of the Drosophila sex determination signal and its link to neurogenesis.
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果蝇性别决定信号的分子性质及其与神经发生的联系。
DOI：
10.1126/science.1900130
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Cline,TW
中科院分区：
文献类型：
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作者：
Erickson,JW;Cline,TW
1921年，人们发现果蝇的性命运是由X染色体与常染色体组的比例决定的。直到最近才发现，X 染色体与常染色体 (X:A) 的比例部分是通过无姐妹-b (sis-b) 的剂量来传达的，无姐妹-b (sis-b) 是一种 X 连锁遗传元件，位于参与神经发生的基因的毛囊-盾复合体中。在本报告中，通过分析sis-b+种系转化体确定了初级性别决定信号的分子性质及其与这些原神经基因的关系。 Thesis-b+功能由蛋白质T4赋予，T4是转录因子螺旋-环-螺旋家族的成员。尽管 T4 与 sis-bandscute-α 共享，但控制性别决定中 T4 表达的 sis-b 的调控区域与控制神经发生中 T4 表达的 scute-α 的调控区域是分开的，并且比它们更简单。 sis依赖性转录因子组装或结合中剂量敏感的协同相互作用可能直接决定性发育的主要调节因子Sex-lethal的雌性特异性启动子的活性。在这个模型中，不需要援引类似的性致死常染色体负调节因子的存在。
In 1921 it was discovered that the sexual fate ofDrosophilais determined by the ratio of X chromosomes to sets of autosomes. Only recently has it been found that the X chromosome to autosome (X:A) ratio is communicated in part by the dose ofsisterless-b(sis-b), an X-linked genetic element located within theachaete-scutecomplex of genes involved in neurogenesis. In this report, the molecular nature of the primary sex determination signal and its relation to these proneural genes was determined by analysis ofsis-b+germline transformants. Thesis-b+function is conferred by protein T4, a member of the helix-loop-helix family of transcription factors. Although T4 is shared bysis-bandscute-α, the regulatory regions ofsis-b, which control T4 expression in sex determination, are both separable from and simpler than those ofscute-α, which control T4 expression in neurogenesis. Dose-sensitive cooperative interactions in the assembly or binding ofsis-dependent transcription factors may directly determine the activity of the female-specific promoter ofSex-lethal, the master regulator of sexual development. In this model there is no need to invoke the existence of analogous autosomal negative regulators ofSex-lethal.