CLINICALLY NONFUNCTIONING PITUITARY-TUMORS ARE MONOCLONAL IN ORIGIN
CLINICALLY NONFUNCTIONING PITUITARY-TUMORS ARE MONOCLONAL IN ORIGIN
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DOI：
10.1172/jci114705
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发表时间：
1990-07-01
影响因子：
15.9
通讯作者：
KLIBANSKI, A
中科院分区：
文献类型：
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作者：
ALEXANDER, JM;BILLER, BMK;KLIBANSKI, A
在临床上，无功能垂体腺瘤是一种良性肿瘤。25%-30%的脑垂体瘤。关于垂体瘤的发病机制，人们知之甚少。克隆分析使人们能够在对刺激因子作出反应的多克隆增殖和遗传异常细胞的单克隆增殖之间做出重要的区分。我们利用磷酸甘油酸激酶和次黄嘌呤磷酸核糖转移酶基因的X-连锁限制性片段长度多态性研究了垂体肿瘤的克隆性起源。应用限制性内切酶分析了6例垂体腺瘤的X等位基因失活模式。所有六种肿瘤都表现出X-失活的单克隆性模式。这些数据表明，无功能垂体腺瘤是单细胞起源的，这一结果与这种肿瘤类型是由体细胞突变引起的假设一致。
Clinically nonfunctioning pituitary adenomas are benign neoplasms comprsing .apprx. 25-30% of pituitary tumors. Little is known about the pathogenesis of pituitary neoplasia. Clonal analysis allows one to make the important distinction between a polyclonal proliferation in response to a stimulatory factor versus a monoclonal expansion of a genetically aberrant cell. We investigated the clonal origin of pituitary tumors using X-linked restriction fragment length polymorphisms at the phosphoglycerate kinase and hypoxanthine phosphoribosyltransferase genes. Restriction enzymes were used to analyze allelic X-inactivation patterns in six pituitary adenomas. All six tumors showed a monoclonal pattern of X-inactivation. These data indicate that nonfunctioning pituitary adenomas are unicellular in origin, a result consistent with the hypothesis that this tumor type is due to somatic mutation.