The effect of adeno-associated virus mediated brain derived neurotrophic factor in an animal model of neurogenic impotence
The effect of adeno-associated virus mediated brain derived neurotrophic factor in an animal model of neurogenic impotence
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DOI：
10.1016/s0022-5347(05)66302-1
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发表时间：
2001-06-01
影响因子：
6.6
通讯作者：
Lue, TF
中科院分区：
文献类型：
--
作者：
Bakircioglu, ME;Lin, CS;Lue, TF
目的：我们测试了用脑源性神经营养因子转染阴茎组织可能促进海绵体神经损伤后神经恢复和勃起能力的假设。 材料和方法：在 34 只 Sprague-Dawley 大鼠中，10 只接受假手术，24 只接受双侧海绵体神经冷冻和海绵体内注射腺相关病毒-lacZ（12）或腺相关病毒-脑源性神经营养因子（12）。在第 4 周和第 8 周时，通过海绵体神经电刺激评估勃起功能，并对阴茎组织和主要骨盆神经节样本进行组织学评估。结果：在第 4 周和第 8 周时，脑源性神经营养因子组的平均最大海绵体内压加或减标准差显着高于 LacZ 组（58.5 +/- 11.7 cm，水 vs 28.4 +/- 5.5 和分别为 61.3 +/- 12.5 与 37.7 +/- 7.9）。此外，在脑源性神经营养因子组中，还原烟酰胺腺嘌呤二核苷酸磷酸心肌黄酶染色和神经元一氧化氮合酶免疫染色显示，在每个时间点，背神经和海绵体组织中的阳性神经纤维明显多于LacZ组，并且盆腔主要神经节中神经元一氧化氮合酶阳性神经元的百分比也显着更高。此外，LacZ组大多数神经元呈浅色染色，轮廓不规则，胞质内有大量空泡。结论：海绵体内注射腺相关病毒脑源性神经营养因子可防止盆腔大神经节含神经元一氧化氮合酶的神经元变性，促进阴茎组织含神经元一氧化氮合酶的神经纤维再生，从而促进双侧海绵体神经术后勃起功能的恢复。受伤。
Purpose: We tested the hypothesis that transfecting penile tissue with brain derived neurotrophic factor may facilitate neural recovery and erectile capability after cavernous nerve injury.Materials and Methods: Of the 34 Sprague-Dawley rats used 10 underwent sham operation and 24 underwent bilateral cavernous nerve freezing and intracavernous injection of adenoassociated virus-lacZ (12) or adeno-associated virus-brain derived neurotrophic factor (12). Erectile function was assessed by cavernous nerve electrostimulation at 4 and 8 weeks, and samples of penile tissue and the major pelvic ganglia were evaluated histologically.Results: In the brain derived neurotrophic factor group mean maximal intracavernous pressure plus or minus standard deviation was significantly higher than in the LacZ group at 4 and 8 weeks (58.5 +/- 11.7 cm, water versus 28.4 +/- 5.5 and 61.3 +/- 12.5 versus 37.7 +/- 7.9, respectively). In addition, in the brain derived neurotrophic factor group reduced nicotinamide adenine dinucleotide phosphate diaphorase staining and neuronal nitric oxide synthase immunostaining revealed significantly more positive nerve fibers in the dorsal nerves and cavernous tissue than in the LacZ group at each time point and the percent of neuronal nitric oxide synthase positive neurons in the major pelvic ganglia was also significantly greater. Moreover, in the LacZ group most neurons showed a light staining pattern with irregular contours and numerous vacuoles in the cytoplasm.Conclusions: Intracavernous injection of adeno-associated virus-brain derived neurotrophic factor may prevent the degeneration of neuronal nitric oxide synthase containing neurons in the major pelvic ganglia and facilitate the regeneration of neuronal nitric oxide synthase containing nerve fibers in penile tissue, thus, enhancing the recovery of erectile function after bilateral cavernous nerve injury.