Insights into Neonatal Oral Feeding through the Salivary Transcriptome.
Insights into Neonatal Oral Feeding through the Salivary Transcriptome.
复制标题
通过唾液转录组深入了解新生儿口腔喂养。
DOI：
10.1155/2012/195153
复制
发表时间：
2012
影响因子：
2.1
通讯作者：
Maron,JillL
中科院分区：
文献类型：
--
作者：
Maron,JillL
背景新生儿安全有效的经口喂养技能的发展是复杂的，可能与重大发病率有关。我们在分子水平上对新生儿经口喂养成熟的理解是有限的，这为利用新兴的分子技术准确评估新生儿经口喂养技能提供了机会。确定新生儿唾液中参与成功口服喂养的关键调控基因。此前，我们的实验室在唾液中发现了9，286个基因，这些基因在早产儿获得先进的口服喂养技能时在统计学上显着改变了它们的基因表达。在这份报告中，以前确定的基因进行了更新和有针对性的途径分析与免疫途径分析（IPA），以确定潜在的候选基因参与成功的口服喂养。如果基因处于五种最显着上调和下调的生理途径中并且与关键词“进食”、“消化”和“发育”相关，则考虑基因。结果。有2,186个基因符合标准。与摄食行为，颅神经发育，神经，骨骼和肌肉系统的发展相关的途径被突出显示。这些数据为在分子水平上了解新生儿经口喂养的生物学过程提供了重要的见解，并确定了与成功经口喂养相关的新途径。
Background. The development of safe and effective oral feeding skills in the newborn is complex and may be associated with significant morbidities. Our understanding of neonatal oral feeding maturation at the molecular level is limited, providing an opportunity to utilize emerging molecular techniques to accurately assess neonatal oral feeding skills.Objective. To identify key regulatory genes in neonatal saliva involved in successful oral feeding.Methods. Previously, our laboratory identified 9,286 genes in saliva that statistically significantly altered their gene expression as premature newborns gained advanced oral feeding skills. In this report, genes previously identified underwent an updated and targeted pathway analysis with Ingenuity Pathway Analysis (IPA) to identify potential candidate genes involved in successful oral feeding. Genes were considered if they were in the five most significantly up‐ and down‐regulated physiological pathways and were associated with the keywords “feeding”, “digestion” and “development”.Results. There were 2,186 genes that met criteria. Pathways associated with feeding behavior, cranial nerve development, and the development of the nervous, skeletal, and muscular systems were highlighted.Discussion. These data provide important insights into the biological processes involved in oral feeding in the newborn at a molecular level and identify novel pathways associated with successful oral feeding.