A distinct population of clonogenic and multipotent murine follicular keratinocytes residing in the upper isthmus
A distinct population of clonogenic and multipotent murine follicular keratinocytes residing in the upper isthmus
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DOI：
10.1242/jcs.025502
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Owens, David M.
中科院分区：
文献类型：
--
作者：
Jensen, Uffe Birk;Yan, Xiaohong;Owens, David M.
成年鼠毛囊膨出区含有具有多能性的表皮干细胞；然而，这些细胞在稳态条件下的有限贡献表明，可能需要额外的干细胞或祖细胞群来维持鳞状和皮脂腺谱系。我们已经发现了一种独特的鼠毛囊角质形成细胞群，存在于上峡（UI），位于漏斗和凸起区域之间，其特征是α 6整合素水平低，CD34和Sca-1呈阴性。纯化的UI细胞产生长期、稳定的表皮、毛囊和皮脂腺细胞系，并能在体内自我更新。这些细胞是非静止的，与鼓包干细胞相比，具有独特的转录谱，可能是表皮干细胞或祖细胞的独特储存库。
The bulge region of adult murine hair follicles harbors epidermal stem cells with multipotent capacity; however, the restricted contributions of these cells under homeostatic conditions indicates that additional stem or progenitor cell populations may be required to maintain squamous and sebaceous lineages. We have identified a distinct population of murine hair follicle keratinocytes residing in the upper isthmus (UI) between the infundibulum and bulge regions that are distinguished by low alpha 6 integrin levels and are negative for CD34 and Sca-1. Purified UI cells give rise to long-term, stable epidermal, follicular and sebaceous lineages and can self-renew in vivo. These cells are non-quiescent and possess a unique transcript profile compared with bulge stem cells and may represent a distinct reservoir of epidermal stem or progenitor cells.