Nucleotide sequence of a cDNA clone encoding mouse protamine 1.
Nucleotide sequence of a cDNA clone encoding mouse protamine 1.
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DOI：
10.1021/bi00324a027
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发表时间：
1985-01
期刊：
影响因子：
2.9
通讯作者：
K. Kleene;R. Distel;N. Hecht
中科院分区：
文献类型：
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作者：
K. Kleene;R. Distel;N. Hecht
已确定编码富含半胱氨酸、含酪氨酸的小鼠鱼精蛋白的404个碱基cDNA的核苷酸序列。该插入片段从小鼠睾丸cDNA文库中分离，编码50个氨基酸的多肽，其中28个是精氨酸，9个是半胱氨酸，3个是酪氨酸。插入片段含有完整的151个碱基的3'非编码区和大部分5'非编码区。小鼠鱼精蛋白1的预测氨基酸序列与公猪鱼精蛋白约80%同源，与公牛鱼精蛋白约67%同源，并且包含在鳟鱼鱼精蛋白中发现的四个精氨酸簇的中心高度碱性结构域。小鼠鱼精蛋白的cDNA克隆的鉴定将有助于研究这种单倍体生精细胞特有的核蛋白的进化、调节和蛋白质-DNA相互作用。
The nucleotide sequence of a 404-base cDNA encoding the cysteine-rich, tyrosine-containing mouse protamine has been determined. This insert, isolated from a mouse testis cDNA library, encodes a polypeptide of 50 amino acids of which 28 are arginine, 9 are cysteine, and 3 are tyrosine. The insert contains the complete 3' noncoding region of 151 bases and most of the 5' noncoding region. The predicted amino acid sequence of mouse protamine 1 is about 80% homologous to boar protamine and 67% homologous to bull protamine and contains the central, highly basic domain of four arginine clusters found in the trout protamines. The identification of a cDNA clone for a mouse protamine will facilitate studies of the evolution, regulation, and protein-DNA interaction of this nuclear protein unique to haploid spermatogenic cells.