DNA hybridization electrochemical sensor using conducting polymer
DNA hybridization electrochemical sensor using conducting polymer
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DOI：
10.1016/s0956-5663(03)00088-55
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发表时间：
2003-09-01
影响因子：
12.6
通讯作者：
Lim, G
中科院分区：
文献类型：
--
作者：
Cha, JH;Han, JI;Lim, G
本文报道了聚噻吩-3-基乙酸1，3-二氧代-1，3-二氢异吲哚-2-基酯（PTAE）在电化学杂交传感器中的应用。介绍了导电聚合物传感器单体噻吩-3-乙酸-1，3-二氧代-1，3-二氢异吲哚-2-酯（TAE）的合成路线。在导电聚合物膜上进行探针寡核苷酸对PTAE中的良好离去基团位点的直接化学取代。生物识别可以通过与单链和双链状态寡核苷酸的电化学信号（循环伏安图）进行比较来监测。电化学传感器的灵敏度为0.62 μ A/nmole，检测限为1 nmole。双链态寡核苷酸的氧化电流约为单链的一半，这与导电聚合物的电化学活性随侧基刚度的增加而降低相对应。完全匹配和单核苷酸错配样品的氧化电流下降率分别为52%和25- 30%。降低的比例越大，归因于单核苷酸错配样品的空间位阻越大。(C)2003 Elsevier Science B. V.保留所有权利。
We report the use of poly(thiophen-3-yl-acetic acid 1,3-dioxo-1,3-dihydro-isoindol-2-yl ester (PTAE) for application to electrochemical hybridization sensor. A synthetic route for the thiophen-3-yl-acetic acid 1,3-dioxo-1,3-dihydro-isoindol-2-yl ester (TAE) is described, which is used as a monomer of conducting polymer sensor. A direct chemical substitution of probe oligonucleotide to good leaving group site in the PTAE is carried out on the conducting polymer film. A biological recognition can be monitored by comparison with the electrochemical signal (cyclic voltammogram) of single and double strand state oligonucleotide. The sensitivity of the electrochemical sensor is 0.62 muA/nmole and the detection limit is 1 nmole. The oxidation current of double strand state oligonucleotide is a half of that of single strand, that is corresponding to the decrease of electrochemical activity of conducting polymer with increase of stiffness of side group of the polymer. The oxidation current decreasing ratios of perfect matched and single nucleotide mismatched samples are 52 and 25-30%, respectively. The more decreasing ratio is attributable to the more steric hindrance of single nucleotide mismatched sample. (C) 2003 Elsevier Science B.V. All rights reserved.