Eugenol protects neuronal cells from excitotoxic and oxidative injury in primary cortical cultures
Eugenol protects neuronal cells from excitotoxic and oxidative injury in primary cortical cultures
复制标题
DOI：
10.1016/s0304-3940(97)00195-x
复制
发表时间：
1997-04-04
影响因子：
2.5
通讯作者：
Kim, YH
中科院分区：
文献类型：
--
作者：
Wie, MB;Won, MH;Kim, YH
我们研究了丁香酚对N-甲基-D-天冬氨酸（NMDA），氧-葡萄糖剥夺，黄嘌呤/黄嘌呤氧化酶诱导的神经毒性在原代小鼠皮层培养的神经保护作用。丁香酚（100-300 μ M）使NMDA（300 μ M）诱导的急性神经毒性减弱20- 60%。在相同的浓度范围内，丁香酚也抑制NMDA（300 μ M）诱导的神经元Ca-45（2+）摄取升高10- 30%。在氧-葡萄糖剥夺（50分钟）的神经毒性，丁香酚（100-300 μ M）防止急性神经元肿胀和减少45-60%的神经元死亡的浓度依赖性的方式。通过加入丁香酚（100-300 μ M），黄嘌呤/黄嘌呤氧化酶诱导的氧化性神经元损伤也显著减少（75-90%）。提示丁香酚可能通过调节NMDA受体和超氧阴离子自由基而发挥抗缺血损伤的作用。(C)1997 Elsevier Science爱尔兰有限公司
We examined the neuroprotective efficacy of eugenol against N-methyl-D-aspartate (NMDA)-, oxygen-glucose deprivation-, and xanthine/xanthine oxidase-induced neurotoxicity in primary murine cortical cultures. Eugenol (100-300 mu M) attenuated NMDA (300 mu M)-induced acute neurotoxicity by 20-60%. At the same concentration range, eugenol also inhibited NMDA (300 mu M)-induced elevation in neuronal Ca-45(2+) uptake by 10-30%. In the oxygen-glucose deprivation (50 min) neurotoxicity, eugenol (100-300 mu M) prevented acute neuronal swelling and reduced neuronal death by 45-60% in a concentration-dependent fashion. Oxidative neuronal injury induced by xanthine/xanthine oxidase was also significantly reduced (75-90%) by eugenol (100-300 mu M) addition. These results suggest that eugenol may play a protective role against ischemic injury by modulating both NMDA receptor and superoxide radical. (C) 1997 Elsevier Science Ireland Ltd.