Brightness-equalized quantum dots.
Brightness-equalized quantum dots.
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DOI：
10.1038/ncomms9210
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发表时间：
2015-10-05
影响因子：
16.6
通讯作者：
Smith AM
中科院分区：
文献类型：
--
作者：
Lim SJ;Zahid MU;Le P;Ma L;Entenberg D;Harney AS;Condeelis J;Smith AM
作为细胞和组织的分子标记，荧光探针塑造了我们对生物结构和过程的理解。然而，它们的定量分析能力是有限的，因为来自多色荧光团的光子发射速率是不同的，不稳定的，并且通常是不可预测的，这模糊了测量的荧光和分子浓度之间的相关性。在这里，我们介绍了一类新的发光量子点，其在广泛的颜色范围内具有可调和均衡的荧光亮度。其主要特征是通过不同的结构域的发射波长，消光系数和量子产率的独立可调谐性在晶体中。精确的调谐消除了100倍的红色到绿色亮度失配的大小调谐量子点在合奏和单粒子水平，这大大提高了定量成像的生物组织的准确性。我们预计，这些材料工程原理将大大扩展荧光探针的光学工程景观，促进定量多色成像活组织和改善发光器件的颜色调谐。 不同尺寸的量子点发射不同波长的光，但亮度也不同，这使得荧光图像的分析变得复杂。在这里，作者合成多色亮度均衡的量子点，通过控制的组成和结构的核壳HgCdSeS-CdZnS纳米晶体。
As molecular labels for cells and tissues, fluorescent probes have shaped our understanding of biological structures and processes. However, their capacity for quantitative analysis is limited because photon emission rates from multicolour fluorophores are dissimilar, unstable and often unpredictable, which obscures correlations between measured fluorescence and molecular concentration. Here we introduce a new class of light-emitting quantum dots with tunable and equalized fluorescence brightness across a broad range of colours. The key feature is independent tunability of emission wavelength, extinction coefficient and quantum yield through distinct structural domains in the nanocrystal. Precise tuning eliminates a 100-fold red-to-green brightness mismatch of size-tuned quantum dots at the ensemble and single-particle levels, which substantially improves quantitative imaging accuracy in biological tissue. We anticipate that these materials engineering principles will vastly expand the optical engineering landscape of fluorescent probes, facilitate quantitative multicolour imaging in living tissue and improve colour tuning in light-emitting devices. Quantum dots with different size emit light at different wavelengths but also different brightness, which complicates analysis of fluorescence images. Here, the authors synthesize multicolour brightness-equalized quantum dots by controlling the composition and structure of core-shell HgCdSeS-CdZnS nanocrystals.