Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection
Isolation of MaDEF from Muscari armeniacum and analysis of its expression using laser microdissection
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DOI：
10.1016/j.plantsci.2005.08.021
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发表时间：
2006-01-01
期刊：
影响因子：
5.2
通讯作者：
Kanno, A
中科院分区：
文献类型：
--
作者：
Nakada, M;Komatsu, M;Kanno, A
在高等真双子叶植物中，花器官由四个不同的轮生体组成，包括萼片、花瓣、雄蕊和心皮。相反，许多单子叶植物的花被，如百合科植物，具有几乎相同的瓣状器官，称为花被片。修改后的ABC模型解释了这种花的形态; B类基因在轮I以及轮2和3中表达，导致两个外轮几乎是相同的瓣状器官。郁金香中B类基因的表达模式支持这一改进的ABC模型，但芦笋B类基因的表达仅限于花被片和雄蕊的内部。为了研究芦笋的B类基因表达模式是否与相关物种共享，从属于天冬目的亚美尼亚蝇中分离了B类基因MaDEF。RT-PCR结果表明，MaDEF不仅在花被片外层表达，而且在花被片内层和雄蕊中也有表达。该表达模式与郁金香的表达模式一致，支持M.杏自从M.为了解决armneniacum花蕾太小而不能准确分离成单个花器官的问题，利用激光显微切割（LMD）系统分离花器官特异性RNA。我们还讨论了适用于植物研究的LMD技术。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
In higher eudicot, the floral organs consist of four different whorls, containing sepals, petals, stamens and carpels. In contrast, the perianths of many monocotyledonous plants, like Liliaceae plants, have almost identical petaloid organs called tepals. The modified ABC model explains this flower morphology; class B genes are expressed in whorl I as well as whorls 2 and 3, resulting that the two outer whorls are almost identical petaloid organs. Expression pattern of class B genes in tulip supports this modified ABC model, however, the expression of asparagus class B genes were restricted in the inner tepals and stamens. To investigate whether the class B gene expression pattern of asparagus is shared by related species, the class B gene MaDEF was isolated from Muscari armeniacum, belonging to the order Asparagales. RT-PCR showed that MaDEF is expressed in outer tepals as well as in inner tepals and stamens. This expression pattern is consistent with that of tulip, supporting the modified ABC model in M. armeniacum. Since M. armneniacum flower buds are too small for accurate separation into individual floral organs, the laser microdissection (LMD) system was used to isolate floral organ-specific RNA. We also discuss the suitability of the LMD technique for plant research. (c) 2005 Elsevier Ireland Ltd. All rights reserved.