Influence of co-culture with denuded oocytes during in vitro maturation on fertilization and developmental competence of cumulus-enclosed porcine oocytes in a defined system
Influence of co-culture with denuded oocytes during in vitro maturation on fertilization and developmental competence of cumulus-enclosed porcine oocytes in a defined system
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DOI：
10.1111/asj.12459
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发表时间：
2016-04-01
影响因子：
2
通讯作者：
Van Soom, Ann
中科院分区：
文献类型：
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作者：
Appeltant, Ruth;Somfai, Tamas;Van Soom, Ann
牛卵丘-卵母细胞复合体（COCs）与裸露卵母细胞（DO）共培养可通过卵母细胞分泌因子提高卵母细胞体外成熟能力。本研究的目的是研究在猪体外成熟过程中添加DO是否可以改善卵母细胞在体外生产系统中的体外受精（IVF）和体外培养（IVC）结果。成熟培养基为猪卵母细胞培养基，添加促性腺激素、dbcAMP和巯基乙醇。卵丘-卵母细胞复合体在无DO或以不同比例（9个COC、9个COC+16个DO和9个COC+36个DO）添加DO的情况下成熟。因此，将卵母细胞作为完整COC或在剥脱后进行IVF，以检查在IVM期间添加DO是否会影响卵丘或卵母细胞性质。受精后，受精卵的穿透率和正常受精率在所有测试组之间没有差异，无论IVF前是否剥脱。当受精卵培养6天时，在所有处理组之间的卵裂率、囊胚率和每个囊胚的细胞数方面没有观察到差异。总之，无论比例如何，在IVM期间与DO共培养并没有改善卵丘封闭的猪卵母细胞在规定系统中的受精参数和胚胎发育。
Co-culture of cumulus-oocyte complexes (COCs) with denuded oocytes (DOs) during in vitro maturation (IVM) was reported to improve the developmental competence of oocytes via oocyte-secreted factors in cattle. The aim of the present study was to investigate if addition of DOs during IVM can improve in vitro fertilization (IVF) and in vitro culture (IVC) results for oocytes in a defined in vitro production system in pigs. The maturation medium was porcine oocyte medium supplemented with gonadotropins, dbcAMP and -mercaptoethanol. Cumulus-oocyte complexes were matured without DOs or with DOs in different ratios (9 COC, 9 COC+16 DO and 9 COC+36 DO). Consequently; oocytes were subjected to IVF as intact COCs or after denudation to examine if DO addition during IVM would affect cumulus or oocyte properties. After fertilization, penetration and normal fertilization rates of zygotes were not different between all tested groups irrespective of denudation before IVF. When zygotes were cultured for 6 days, no difference could be observed between all treatment groups in cleavage rate, blastocyst rate and cell number per blastocyst. In conclusion, irrespective of the ratio, co-culture with DOs during IVM did not improve fertilization parameters and embryo development of cumulus-enclosed porcine oocytes in a defined system.