A highly divergent Puumala virus lineage in southern Poland
A highly divergent Puumala virus lineage in southern Poland
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DOI：
10.1007/s00705-016-3200-5
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发表时间：
2017-05-01
影响因子：
2.7
通讯作者：
Ulrich, Rainer G.
中科院分区：
文献类型：
--
作者：
Rosenfeld, Ulrike M.;Drewes, Stephan;Ulrich, Rainer G.
普马拉病毒（PUUV）是中欧最重要的汉坦病毒之一。puv毒株的系统发育分析表明该汉坦病毒具有很强的遗传结构。最近，在波兰北部采集的银行田鼠（Myodes glareolus）天然储层中发现了PUUV序列。本研究的目的是评估PUUV在波兰南部银行田鼠中的存在。2009年，在波兰这一地区的6个地点共捕获了72只田鼠。RT-PCR和IgG-ELISA分析在一个诱捕点检测到3只PUUV阳性田鼠。经细胞色素b基因分析，puuv感染鼠属喀尔巴阡和东方河岸田鼠进化谱系。新的PUUV S、M和L片段核苷酸序列与来自拉脱维亚的俄罗斯PUUV谱系序列最相似，但与先前在波兰北部、斯洛伐克和奥地利发现的PUUV序列高度不同。总之，在波兰南部检测到高度分化的puv谱系表明有必要在该地区进一步监测田鼠，以便采取合理的公共卫生措施预防人类感染。
Puumala virus (PUUV) represents one of the most important hantaviruses in Central Europe. Phylogenetic analyses of PUUV strains indicate a strong genetic structuring of this hantavirus. Recently, PUUV sequences were identified in the natural reservoir, the bank vole (Myodes glareolus), collected in the northern part of Poland. The objective of this study was to evaluate the presence of PUUV in bank voles from southern Poland. A total of 72 bank voles were trapped in 2009 at six sites in this part of Poland. RT-PCR and IgG-ELISA analyses detected three PUUV positive voles at one trapping site. The PUUV-infected animals were identified by cytochrome b gene analysis to belong to the Carpathian and Eastern evolutionary lineages of bank vole. The novel PUUV S, M and L segment nucleotide sequences showed the closest similarity to sequences of the Russian PUUV lineage from Latvia, but were highly divergent to those previously found in northern Poland, Slovakia and Austria. In conclusion, the detection of a highly divergent PUUV lineage in southern Poland indicates the necessity of further bank vole monitoring in this region allowing rational public health measures to prevent human infections.